Single Cell GFP-Trap Reveals Stoichiometry and Dynamics of Cytosolic Protein Complexes

DC FieldValueLanguage
dc.contributor.authorWedeking, Tim
dc.contributor.authorLoechte, Sara
dc.contributor.authorRichter, Christian P.
dc.contributor.authorBhagawati, Maniraj
dc.contributor.authorPiehier, Jacob
dc.contributor.authorYou, Changjiang
dc.date.accessioned2021-12-23T15:58:38Z-
dc.date.available2021-12-23T15:58:38Z-
dc.date.issued2015
dc.identifier.issn15306984
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/3492-
dc.description.abstractWe developed in situ single cell pull-down (SiCPull) of GFP-tagged protein complexes based on micropatterned functionalized surface architectures. Cells cultured on these supports are lysed by mild detergents and protein complexes captured to the surface are probed in situ by total internal reflection fluorescence microscopy. Using SiCPull, we quantitatively mapped the lifetimes of various signal transducer and activator of transcription complexes by monitoring dissociation from the surface and defined their stoichiometry on the single molecule level.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; We thank Gabriele Hikade and Hella Kenneweg for technical support and Rainer Kurre for his support with fluorescence microscopy. This project was supported by the Deutsche Forschungsgemeinschaft (SFB 944).
dc.language.isoen
dc.publisherAMER CHEMICAL SOC
dc.relation.ispartofNANO LETTERS
dc.subjectASSOCIATION
dc.subjectChemistry
dc.subjectChemistry, Multidisciplinary
dc.subjectChemistry, Physical
dc.subjectfunctional surface micropatterning
dc.subjectLIVE CELLS
dc.subjectLIVING CELLS
dc.subjectMASS-SPECTROMETRY
dc.subjectMaterials Science
dc.subjectMaterials Science, Multidisciplinary
dc.subjectMOLECULE PULL-DOWN
dc.subjectMONOFUNCTIONALIZATION
dc.subjectnanobody
dc.subjectNanoscience & Nanotechnology
dc.subjectPhysics
dc.subjectPhysics, Applied
dc.subjectPhysics, Condensed Matter
dc.subjectPLASMA-MEMBRANE
dc.subjectPREASSOCIATION
dc.subjectProtein-protein interaction
dc.subjectScience & Technology - Other Topics
dc.subjectsignal transducer and activator of transcription
dc.subjectSIGNALING COMPLEXES
dc.subjectsingle molecule imaging
dc.subjectSTAT1
dc.titleSingle Cell GFP-Trap Reveals Stoichiometry and Dynamics of Cytosolic Protein Complexes
dc.typejournal article
dc.identifier.doi10.1021/acs.nanolett.5b01153
dc.identifier.isiISI:000354906000125
dc.description.volume15
dc.description.issue5
dc.description.startpage3610
dc.description.endpage3615
dc.contributor.orcid0000-0002-7839-6397
dc.contributor.researcheridL-3901-2014
dc.identifier.eissn15306992
dc.publisher.place1155 16TH ST, NW, WASHINGTON, DC 20036 USA
dcterms.isPartOf.abbreviationNano Lett.
crisitem.author.deptSonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente-
crisitem.author.deptidorganisation19-
crisitem.author.orcid0000-0002-7839-6397-
crisitem.author.parentorgFB 05 - Biologie/Chemie-
crisitem.author.grandparentorgUniversität Osnabrück-
crisitem.author.netidYoCh745-
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