Single Cell GFP-Trap Reveals Stoichiometry and Dynamics of Cytosolic Protein Complexes
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Wedeking, Tim | |
dc.contributor.author | Loechte, Sara | |
dc.contributor.author | Richter, Christian P. | |
dc.contributor.author | Bhagawati, Maniraj | |
dc.contributor.author | Piehier, Jacob | |
dc.contributor.author | You, Changjiang | |
dc.date.accessioned | 2021-12-23T15:58:38Z | - |
dc.date.available | 2021-12-23T15:58:38Z | - |
dc.date.issued | 2015 | |
dc.identifier.issn | 15306984 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/3492 | - |
dc.description.abstract | We developed in situ single cell pull-down (SiCPull) of GFP-tagged protein complexes based on micropatterned functionalized surface architectures. Cells cultured on these supports are lysed by mild detergents and protein complexes captured to the surface are probed in situ by total internal reflection fluorescence microscopy. Using SiCPull, we quantitatively mapped the lifetimes of various signal transducer and activator of transcription complexes by monitoring dissociation from the surface and defined their stoichiometry on the single molecule level. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 944]; We thank Gabriele Hikade and Hella Kenneweg for technical support and Rainer Kurre for his support with fluorescence microscopy. This project was supported by the Deutsche Forschungsgemeinschaft (SFB 944). | |
dc.language.iso | en | |
dc.publisher | AMER CHEMICAL SOC | |
dc.relation.ispartof | NANO LETTERS | |
dc.subject | ASSOCIATION | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | Chemistry, Physical | |
dc.subject | functional surface micropatterning | |
dc.subject | LIVE CELLS | |
dc.subject | LIVING CELLS | |
dc.subject | MASS-SPECTROMETRY | |
dc.subject | Materials Science | |
dc.subject | Materials Science, Multidisciplinary | |
dc.subject | MOLECULE PULL-DOWN | |
dc.subject | MONOFUNCTIONALIZATION | |
dc.subject | nanobody | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | Physics | |
dc.subject | Physics, Applied | |
dc.subject | Physics, Condensed Matter | |
dc.subject | PLASMA-MEMBRANE | |
dc.subject | PREASSOCIATION | |
dc.subject | Protein-protein interaction | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | signal transducer and activator of transcription | |
dc.subject | SIGNALING COMPLEXES | |
dc.subject | single molecule imaging | |
dc.subject | STAT1 | |
dc.title | Single Cell GFP-Trap Reveals Stoichiometry and Dynamics of Cytosolic Protein Complexes | |
dc.type | journal article | |
dc.identifier.doi | 10.1021/acs.nanolett.5b01153 | |
dc.identifier.isi | ISI:000354906000125 | |
dc.description.volume | 15 | |
dc.description.issue | 5 | |
dc.description.startpage | 3610 | |
dc.description.endpage | 3615 | |
dc.contributor.orcid | 0000-0002-7839-6397 | |
dc.contributor.researcherid | L-3901-2014 | |
dc.identifier.eissn | 15306992 | |
dc.publisher.place | 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | |
dcterms.isPartOf.abbreviation | Nano Lett. | |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.orcid | 0000-0002-7839-6397 | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | YoCh745 | - |
Seitenaufrufe
2
Letzte Woche
1
1
Letzter Monat
1
1
geprüft am 09.06.2024