New yeast/E. coli/Drosophila triple shuttle vectors for efficient generation of Drosophila P element transformation constructs

DC FieldValueLanguage
dc.contributor.authorPaululat, Achim
dc.contributor.authorHeinisch, Juergen J.
dc.date.accessioned2021-12-23T15:58:41Z-
dc.date.available2021-12-23T15:58:41Z-
dc.date.issued2012
dc.identifier.issn03781119
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/3507-
dc.description.abstractWe have generated a set of novel triple shuttle vectors that facilitate the construction of Drosophila-P-element transformations vectors. These YED-vectors allow the insertion of any kind of sequence at any chosen position due to the presence of a yeast casette which ensures replication and allows for homologous recombination in Saccharomyces cerevisiae. As a proof of principle we generated several reporter constructs and tested them in transgenic flies for expression and correct subcellular localization. YED-vectors can be used for many purposes including promoter analysis or the expression of tagged or truncated proteins. Thus, time-consuming conventional restriction site based multi-step cloning procedures can be circumvented by using the new YED-vectors. The new set of triple shuttle vectors will be highly beneficial for the rapid construction of complex Drosophila transformation plasmids. (C) 2012 Elsevier B.V. All rights reserved,
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofGENE
dc.subjectDrosophila
dc.subjectE. coli
dc.subjectEXPRESSION
dc.subjectFRAGMENTS
dc.subjectGENETIC-TRANSFORMATION
dc.subjectGenetics & Heredity
dc.subjectHOMOLOGOUS RECOMBINATION
dc.subjectMELANOGASTER
dc.subjectP-element
dc.subjectPHI-C31
dc.subjectTriple shuttle vector
dc.subjectYeast
dc.titleNew yeast/E. coli/Drosophila triple shuttle vectors for efficient generation of Drosophila P element transformation constructs
dc.typejournal article
dc.identifier.doi10.1016/j.gene.2012.09.058
dc.identifier.isiISI:000313024700023
dc.description.volume511
dc.description.issue2
dc.description.startpage300
dc.description.endpage305
dc.contributor.orcid0000-0002-8845-6859
dc.contributor.researcheridG-3801-2017
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationGene
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-8845-6859-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPaAc947-
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