New yeast/E. coli/Drosophila triple shuttle vectors for efficient generation of Drosophila P element transformation constructs
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Paululat, Achim | |
dc.contributor.author | Heinisch, Juergen J. | |
dc.date.accessioned | 2021-12-23T15:58:41Z | - |
dc.date.available | 2021-12-23T15:58:41Z | - |
dc.date.issued | 2012 | |
dc.identifier.issn | 03781119 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/3507 | - |
dc.description.abstract | We have generated a set of novel triple shuttle vectors that facilitate the construction of Drosophila-P-element transformations vectors. These YED-vectors allow the insertion of any kind of sequence at any chosen position due to the presence of a yeast casette which ensures replication and allows for homologous recombination in Saccharomyces cerevisiae. As a proof of principle we generated several reporter constructs and tested them in transgenic flies for expression and correct subcellular localization. YED-vectors can be used for many purposes including promoter analysis or the expression of tagged or truncated proteins. Thus, time-consuming conventional restriction site based multi-step cloning procedures can be circumvented by using the new YED-vectors. The new set of triple shuttle vectors will be highly beneficial for the rapid construction of complex Drosophila transformation plasmids. (C) 2012 Elsevier B.V. All rights reserved, | |
dc.language.iso | en | |
dc.publisher | ELSEVIER SCIENCE BV | |
dc.relation.ispartof | GENE | |
dc.subject | Drosophila | |
dc.subject | E. coli | |
dc.subject | EXPRESSION | |
dc.subject | FRAGMENTS | |
dc.subject | GENETIC-TRANSFORMATION | |
dc.subject | Genetics & Heredity | |
dc.subject | HOMOLOGOUS RECOMBINATION | |
dc.subject | MELANOGASTER | |
dc.subject | P-element | |
dc.subject | PHI-C31 | |
dc.subject | Triple shuttle vector | |
dc.subject | Yeast | |
dc.title | New yeast/E. coli/Drosophila triple shuttle vectors for efficient generation of Drosophila P element transformation constructs | |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.gene.2012.09.058 | |
dc.identifier.isi | ISI:000313024700023 | |
dc.description.volume | 511 | |
dc.description.issue | 2 | |
dc.description.startpage | 300 | |
dc.description.endpage | 305 | |
dc.contributor.orcid | 0000-0002-8845-6859 | |
dc.contributor.researcherid | G-3801-2017 | |
dc.publisher.place | PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS | |
dcterms.isPartOf.abbreviation | Gene | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-8845-6859 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | PaAc947 | - |
Seitenaufrufe
3
Letzte Woche
0
0
Letzter Monat
2
2
geprüft am 08.06.2024