Three-stepped rotation of subunits gamma and epsilon in single molecules of F-ATPase as revealed by polarized, confocal fluorometry

DC FieldValueLanguage
dc.contributor.authorHasler, K
dc.contributor.authorEngelbrecht, S
dc.contributor.authorJunge, W
dc.date.accessioned2021-12-23T15:58:47Z-
dc.date.available2021-12-23T15:58:47Z-
dc.date.issued1998
dc.identifier.issn00145793
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/3562-
dc.description.abstractThe proton translocating ATP synthase is conceived as a rotatory molecular engine. ATP hydrolysis by its headpiece, CF1, drives the rotation of subunit gamma relative to the hexagonally arranged large subunits, (alpha beta)(3). We investigated transition states of the rotatory drive by polarized confocal fluorometry (POCOF) as applied to single molecules of engineered, immobilized and load-free spinach-CF1. We found that the hydrolysis of ATP caused the stepped and sequential progression of subunit gamma through three discrete angular positions, with the transition states of gamma being too shortlived for detection. We also observed the stepped motion of epsilon, whereas delta was immobile as (alpha beta)(3). (C) 1998 Federation of European Biochemical Societies.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofFEBS LETTERS
dc.subjectATP synthase
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCATALYTIC SITES
dc.subjectCell Biology
dc.subjectCHLOROPLAST
dc.subjectDELTA-SUBUNIT
dc.subjectESCHERICHIA-COLI F1-ATPASE
dc.subjectmolecular motor
dc.subjectRECONSTITUTION
dc.subjectrelation
dc.subjectsingle molecule
dc.subjectSYNTHASE
dc.titleThree-stepped rotation of subunits gamma and epsilon in single molecules of F-ATPase as revealed by polarized, confocal fluorometry
dc.typejournal article
dc.identifier.doi10.1016/S0014-5793(98)00358-5
dc.identifier.isiISI:000073508900003
dc.description.volume426
dc.description.issue3
dc.description.startpage301
dc.description.endpage304
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationFEBS Lett.
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidJuWo587-
Show simple item record

Google ScholarTM

Check

Altmetric