A trimeric metazoan Rab7 GEF complex is crucial for endocytosis and scavenger function
DC Element | Wert | Sprache |
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dc.contributor.author | Dehnen, Lena | |
dc.contributor.author | Janz, Maren | |
dc.contributor.author | Verma, Jitender Kumar | |
dc.contributor.author | Psathaki, Olympia Ekaterini | |
dc.contributor.author | Langemeyer, Lars | |
dc.contributor.author | Frohlich, Florian | |
dc.contributor.author | Heinisch, Jurgen J. | |
dc.contributor.author | Meyer, Heiko | |
dc.contributor.author | Ungermann, Christian | |
dc.contributor.author | Paululat, Achim | |
dc.date.accessioned | 2021-12-23T15:58:53Z | - |
dc.date.available | 2021-12-23T15:58:53Z | - |
dc.date.issued | 2020 | |
dc.identifier.issn | 00219533 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/3630 | - |
dc.description.abstract | Endosome biogenesis in eukaryotic cells is critical for nutrient uptake and plasma membrane integrity. Early endosomes initially contain Rab5, which is replaced by Rab7 on late endosomes prior to their fusion with lysosomes. Recruitment of Rab7 to endosomes requires the Mon1-Ccz1 guanine-nucleotide-exchange factor (GEF). Here, we show that full function of the Drosophila Mon1-Ccz1 complex requires a third stoichiometric subunit, termed Bulli (encoded by CG8270). Bulli localises to Rab7-positive endosomes, in agreement with its function in the GEF complex. Using Drosophila nephrocytes as a model system, we observe that absence of Bulli results in (i) reduced endocytosis, (ii) Rab5 accumulation within non-acidified enlarged endosomes, (iii) defective Rab7 localisation and (iv) impaired endosomal maturation. Moreover, longevity of animals lacking bulli is affected. Both the Mon1-Ccz1 dimer and a Bullicontaining trimer display Rab7 GEF activity. In summary, this suggests a key role for Bulli in the Rab5 to Rab7 transition during endosomal maturation rather than a direct influence on the GEF activity of Mon1-Ccz1. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [PA 517/12-1, SFB 944 TP7, SFB944, UN 111/9-1, UN 111/10-1, SFB 944 TP11, SFB 944 TP21, FR 3647/2-1, SFB 944 TP20]; Deutscher Akademischer Austauschdienst (DAAD)Deutscher Akademischer Austausch Dienst (DAAD); State of Lower SaxonyEuropean Commission [ZN2832]; This work was supported by grants from the Deutsche Forschungsgemeinschaft (PA 517/12-1, SFB 944 TP7, SFB944 Z-Project), the Deutscher Akademischer Austauschdienst (DAAD) and the State of Lower Saxony (ZN2832) to A.P., and by grants from the Deutsche Forschungsgemeinschaft to C.U. (UN 111/9-1, UN 111/10-1, SFB 944 TP11, SFB944 Z-Project), to H.M. (SFB 944 TP21) and to F.F. (FR 3647/2-1, SFB 944 TP20). | |
dc.language.iso | en | |
dc.publisher | COMPANY BIOLOGISTS LTD | |
dc.relation.ispartof | JOURNAL OF CELL SCIENCE | |
dc.subject | Cell Biology | |
dc.subject | Drosophila | |
dc.subject | DROSOPHILA-MELANOGASTER | |
dc.subject | ENDOSOME | |
dc.subject | GARLAND CELL | |
dc.subject | GEF | |
dc.subject | GTPASE YPT7 | |
dc.subject | IDENTIFICATION | |
dc.subject | LYSOSOME | |
dc.subject | Mon1-Ccz1 complex | |
dc.subject | MON1-CCZ1 GEF | |
dc.subject | NEPHROCYTES | |
dc.subject | PERICARDIAL CELLS | |
dc.subject | ULTRASTRUCTURE | |
dc.title | A trimeric metazoan Rab7 GEF complex is crucial for endocytosis and scavenger function | |
dc.type | journal article | |
dc.identifier.doi | 10.1242/jcs.247080 | |
dc.identifier.isi | ISI:000561040700027 | |
dc.description.volume | 133 | |
dc.description.issue | 13 | |
dc.contributor.orcid | 0000-0002-4309-0910 | |
dc.contributor.orcid | 0000-0002-8845-6859 | |
dc.contributor.orcid | 0000-0001-8307-2189 | |
dc.identifier.eissn | 14779137 | |
dc.publisher.place | BIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND | |
dcterms.isPartOf.abbreviation | J. Cell Sci. | |
dcterms.oaStatus | hybrid | |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | Sonderforschungsbereich 944: Physiologie und Dynamik zellulärer Mikrokompartimente | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | organisation19 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.orcid | 0000-0002-4035-6840 | - |
crisitem.author.orcid | 0000-0001-8307-2189 | - |
crisitem.author.orcid | 0000-0002-8845-6859 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | FB 05 - Biologie/Chemie | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.grandparentorg | Universität Osnabrück | - |
crisitem.author.netid | PsOl764 | - |
crisitem.author.netid | FrFl166 | - |
crisitem.author.netid | UnCh999 | - |
crisitem.author.netid | PaAc947 | - |
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geprüft am 15.05.2024