Multiplexing with SERS labels using mixed SAMs of Raman reporter molecules

DC FieldValueLanguage
dc.contributor.authorGellner, Magdalena
dc.contributor.authorKoempe, Karsten
dc.contributor.authorSchluecker, Sebastian
dc.date.accessioned2021-12-23T15:58:58Z-
dc.date.available2021-12-23T15:58:58Z-
dc.date.issued2009
dc.identifier.issn16182642
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/3668-
dc.description.abstractSurface-enhanced Raman scattering (SERS) offers a tremendous multiplexing capacity for the selective detection of biomolecules in targeted research. SERS labels comprising self-assembled monolayers (SAMs) of Raman reporter molecules on the surface of metal nanoparticles are sensitive and robust probes. Advantages of a SAM include maximum sensitivity, minimal unwanted co-adsorption of molecules from the surroundings, and reproducible SERS spectra with only few dominant Raman bands-all of these independent of a particular SERS substrate. We demonstrate experimentally how to increase the multiplexing capacity of SERS labels by using mixed SAMs with up to three different Raman reporter molecules on the surface of the metal colloid. Type and stoichiometry of a particular Raman label in a multi-component SAM are additional parameters compared with one-component SAMs. All one-, two-, and three-component SAMs on gold nanospheres can be easily discriminated, either by their original SERS spectra or the corresponding bar codes.
dc.description.sponsorshipGerman Research FoundationGerman Research Foundation (DFG) [SFB 630/TP C1, SCHL 594/4-1]; We thank the German Research Foundation (DFG; SFB 630/TP C1, SCHL 594/4-1 and Heisenberg fellowship for S. S.) and the Free State of Bavaria (FLUGGE for M. G.) for ongoing financial support of our work. We are grateful to Roche Diagnostics (Drs. T. Froehlich, T. Heckel, D. Heindl, H.-P. Josel, and K. Kurzinger) for providing the gold nanoparticles, B. Kustner and S. Niebling for the least square fit routine in Matlab, M. Schutz for helpful comments, and Prof. Dr. M. Haase for access to his high-resolution TEM.
dc.language.isoen
dc.publisherSPRINGER HEIDELBERG
dc.relation.ispartofANALYTICAL AND BIOANALYTICAL CHEMISTRY
dc.subjectBiochemical Research Methods
dc.subjectBiochemistry & Molecular Biology
dc.subjectChemistry
dc.subjectChemistry, Analytical
dc.subjectGold
dc.subjectMultiplexing
dc.subjectNanoparticle
dc.subjectSelf-assembled monolayer
dc.subjectSurface-enhanced Raman scattering
dc.titleMultiplexing with SERS labels using mixed SAMs of Raman reporter molecules
dc.typejournal article
dc.identifier.doi10.1007/s00216-009-2868-8
dc.identifier.isiISI:000268000600014
dc.description.volume394
dc.description.issue7
dc.description.startpage1839
dc.description.endpage1844
dc.contributor.orcid0000-0003-4790-4616
dc.contributor.researcheridD-1031-2010
dc.identifier.eissn16182650
dc.publisher.placeTIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
dcterms.isPartOf.abbreviationAnal. Bioanal. Chem.
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