A NOTE ON THE IDENTITY OF PORCINE LIVER CARBOXYLESTERASE AND PROLYL-BETA-NAPHTHYLAMIDASE

Autor(en): HEYMANN, E
PETER, K
Stichwörter: Biochemistry & Molecular Biology; CARBOXYLESTERASE ISOENZYMES; ENZYME; ESTERASE; ISOENZYMES; MICROSOMES; PIG LIVER; PROLYL-BETA-NAPHTHYLAMIDASE; PURIFICATION
Erscheinungsdatum: 1993
Herausgeber: WALTER DE GRUYTER & CO
Journal: BIOLOGICAL CHEMISTRY HOPPE-SEYLER
Volumen: 374
Ausgabe: 11
Startseite: 1033
Seitenende: 1036
Zusammenfassung: 
Prolyl-beta-naphthylamidase from porcine liver is compared with the two prevalent isoenzymes of pig liver carboxylesterase by isoelectrofocusing experiments and by inhibition studies with phenyl-methyl-sulfonyl fluoride. The results suggest that prolyl-beta-naphthylamidase is identical with the amide-cleaving isoenzyme of carboxylesterase, not with the usually predominant methyl butyrate-hydrolysing isoenzyme. It is questionable whether the recently published sequence of prolyl-beta-naphthylamidase does belong to this enzyme or to the predominant carboxylesterase without amidase activity. Surprisingly, the amide-cleaving carboxylesterase isoenzymes from rat liver have almost no activity with prolyl-beta-naphthylamide.
ISSN: 01773593
DOI: 10.1515/bchm3.1993.374.7-12.1033

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