Assembly of the Escherichia coli FoF1 ATP synthase involves distinct subcomplex formation

Autor(en): Deckers-Hebestreit, Gabriele 
Stichwörter: ADENOSINE-TRIPHOSPHATASE; ALPHA-SUBUNIT; assembly; ATP synthase; ATPase; BETA-SUBUNIT; Biochemistry & Molecular Biology; biogenesis; DELTA-SUBUNIT; EPSILON-SUBUNIT; Escherichia coli; F1F0-ATP SYNTHASE; FoF1 ATP synthase; MEMBRANE INSERTION; MOTIVE FORCE; PROTON-TRANSLOCATING ATPASE; SUBUNIT-C
Erscheinungsdatum: 2013
Herausgeber: PORTLAND PRESS LTD
Journal: BIOCHEMICAL SOCIETY TRANSACTIONS
Volumen: 41
Ausgabe: 5
Startseite: 1288
Seitenende: 1293
Zusammenfassung: 
The ATP synthase (FoF1) of Escherichia coli couples the translocation of protons across the cytoplasmic membrane by F-o to ATP synthesis or hydrolysis in F-1. Whereas good knowledge of the nanostructure and the rotary mechanism of the ATP synthase is at hand, the assembly pathway of the 22 polypeptide chains present in a stoichiometry of ab(2)c(10)alpha(3)beta(3)gamma delta epsilon. de has so far not received sufficient attention. In our studies, mutants that synthesize different sets of FoF1 subunits allowed the characterization of individually formed stable subcomplexes. Furthermore, the development of a time-delayed in vivo assembly system enabled the subsequent synthesis of particular missing subunits to allow the formation of functional ATP synthase complexes. These observations form the basis for a model that describes the assembly pathway of the E. coli ATP synthase from pre-formed subcomplexes, thereby avoiding membrane proton permeability by a concomitant assembly of the open H+ - translocating unit within a coupled FoF1 complex.
ISSN: 03005127
DOI: 10.1042/BST20130096

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