Evaluation of nanoparticles as endocytic tracers in cellular microbiology

Autor(en): Zhang, Yuying
Hensel, Michael 
Stichwörter: CELLS; Chemistry; Chemistry, Multidisciplinary; GOLD NANOPARTICLES; INTRACELLULAR TRAFFICKING; Materials Science; Materials Science, Multidisciplinary; Nanoscience & Nanotechnology; Physics; Physics, Applied; Science & Technology - Other Topics; SILICA NANOPARTICLES; SIZE-DEPENDENT CYTOTOXICITY; SURFACE-CHARGE; SYSTEM
Erscheinungsdatum: 2013
Herausgeber: ROYAL SOC CHEMISTRY
Journal: NANOSCALE
Volumen: 5
Ausgabe: 19
Startseite: 9296
Seitenende: 9309
Zusammenfassung: 
The study of pathogen interactions with eukaryotic host cells requires the introduction of fluorescent probes to visualize processes such as endocytosis, intracellular transport or host cell manipulation by the pathogen. Here, three types of fluorescent nanoparticles (NPs), i.e. Rhodamine-labeled polymethacrylate (PMA) NPs, silica NPs and gold NPs, were employed to label the host cellular endolysosomal system and monitor manipulations by the pathogen Salmonella enterica. Using live cell imaging, we investigated the performance of NPs in cellular uptake, labeling of endocytic vesicles and lysosomes, as well as interaction with the pathogen. We show that fluorescent gold and silica, but not PMA NPs appropriately label host cell structures and efficiently track rearrangements of the host endosomal system by the activities of intracellular Salmonella. Silica NPs slightly aggregated and located in Salmonella-induced compartments as isolated dots, while gold NPs distributed uniformly inside such structures. Both silica and gold NPs exhibited no adverse impact on either host cells or pathogens, and are versatile tools for infection biology.
ISSN: 20403364
DOI: 10.1039/c3nr01550e

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