HOOGSTEEN-DUPLEX DNA - SYNTHESIS AND BASE-PAIRING OF OLIGONUCLEOTIDES CONTAINING 1-DEAZA-2'-DEOXYADENOSINE
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | SEELA, F | |
dc.contributor.author | WENZEL, T | |
dc.date.accessioned | 2021-12-23T15:59:21Z | - |
dc.date.available | 2021-12-23T15:59:21Z | - |
dc.date.issued | 1994 | |
dc.identifier.issn | 0018019X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/3879 | - |
dc.description.abstract | Oligodeoxyribonucleotides containing 1-deaza-2'-deoxyadenosine (= 7-amino-3-(2-deoxy-beta-D-erythro-pentofuranosyl)-3H-imidazo[4, 5-b]pyridine; 1b) form Hoogsteen duplexes. Watson-Crick base pairs cannot be built up due to the absence of N(1). For these studies, oligonucleotide building blocks - the phosphonate 3a and the phosphoramidite 3b - were prepared from 1b via 4a and 5, as well as the Fractosil-linked 6b, and used in solid-phase synthesis. The applicability of various N-protecting groups (see 4a-c) was also studied. The Hoogsteen duplex d[(c(1)A)(20].d(T?(20)) (11.13; T-m 15 degrees) is less stable than d(A(2)0).d(T-20) (12.13; T-m 60 degrees). The block oligomers d[(c(1)A)(10)-T-10] (14 and d[T-10-(c(1)A)(10] (15) containing purine and pyrimidine bases in the same strand are also able to form duplexes with each other. The chain polarity was found to be parallel. | |
dc.language.iso | en | |
dc.publisher | NEW SWISS CHEMICAL SOC | |
dc.relation.ispartof | HELVETICA CHIMICA ACTA | |
dc.subject | ADENOSINE-DEAMINASE | |
dc.subject | BUILDING-BLOCKS | |
dc.subject | Chemistry | |
dc.subject | Chemistry, Multidisciplinary | |
dc.subject | D(T)N.D(A)N.D(T)N | |
dc.subject | DEOXYNUCLEOSIDES | |
dc.subject | DEOXYRIBONUCLEOSIDES | |
dc.subject | OLIGODEOXYRIBONUCLEOTIDES | |
dc.subject | PHOSPHORAMIDITE | |
dc.subject | RIBONUCLEOSIDES | |
dc.subject | SOLID-PHASE SYNTHESIS | |
dc.subject | TRIPLE-HELIX FORMATION | |
dc.title | HOOGSTEEN-DUPLEX DNA - SYNTHESIS AND BASE-PAIRING OF OLIGONUCLEOTIDES CONTAINING 1-DEAZA-2'-DEOXYADENOSINE | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/hlca.19940770604 | |
dc.identifier.isi | ISI:A1994PH97600003 | |
dc.description.volume | 77 | |
dc.description.issue | 6 | |
dc.description.startpage | 1485 | |
dc.description.endpage | 1499 | |
dc.publisher.place | VERLAG HELVETICA CHIMICA ACTA, MALZGASSE 21, POSTFACH 313, CH-4010 BASEL, SWITZERLAND | |
dcterms.isPartOf.abbreviation | Helv. Chim. Acta |
Seitenaufrufe
4
Letzte Woche
0
0
Letzter Monat
2
2
geprüft am 01.06.2024