Voltages and resistances of the anterior Malpighian tubule of Drosophila melanogaster

DC FieldValueLanguage
dc.contributor.authorBeyenbach, Klaus W.
dc.date.accessioned2021-12-23T15:59:27Z-
dc.date.available2021-12-23T15:59:27Z-
dc.date.issued2019
dc.identifier.issn00220949
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/3936-
dc.description.abstractThe small size of Malpighian tubules in the fruit fly Drosophila melanogaster has discouraged measurements of the transepithelial electrical resistance. The present study introduces two methods for measuring the transepithelial resistance in isolated D. melanogaster Malpighian tubules using conventional microelectrodes and PClamp hardware and software. The first method uses three microelectrodes to measure the specific transepithelial resistance normalized to tubule length or luminal surface area for comparison with resistances of other epithelia. The second method uses only two microelectrodes to measure the relative resistance for comparing before and after effects in a single Malpighian tubule. Knowledge of the specific transepithelial resistance allows the first electrical model of electrolyte secretion by the main segment of the anterior Malpighian tubule of D. melanogaster. The electrical model is remarkably similar to that of the distal Malpighian tubule of Aedes aegypti when tubules of Drosophila and Aedes are studied in vitro under the same experimental conditions. Thus, despite 189 millions of years of evolution separating these two genera, the electrophysiological properties of their Malpighian tubules remains remarkably conserved.
dc.language.isoen
dc.publisherCOMPANY BIOLOGISTS LTD
dc.relation.ispartofJOURNAL OF EXPERIMENTAL BIOLOGY
dc.subjectAEDES-AEGYPTI
dc.subjectBASOLATERAL ION-TRANSPORT
dc.subjectBiology
dc.subjectCable analysis
dc.subjectCATION-TRANSPORT
dc.subjectCHLORIDE CHANNELS
dc.subjectDiuretics
dc.subjectELECTRICAL-RESISTANCE
dc.subjectElectrogenic transport
dc.subjectEpithelial electrophysiology
dc.subjectFLUID SECRETION
dc.subjectFUNCTIONAL-CHARACTERIZATION
dc.subjectH+-ATPASE
dc.subjectKinins
dc.subjectLife Sciences & Biomedicine - Other Topics
dc.subjectRENAL TUBULES
dc.subjectSTELLATE CELLS
dc.titleVoltages and resistances of the anterior Malpighian tubule of Drosophila melanogaster
dc.typejournal article
dc.identifier.doi10.1242/jeb.201574
dc.identifier.isiISI:000469868900017
dc.description.volume222
dc.description.issue10
dc.contributor.orcid0000-0003-3652-2102
dc.identifier.eissn14779145
dc.publisher.placeBIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND
dcterms.isPartOf.abbreviationJ. Exp. Biol.
dcterms.oaStatushybrid
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