Spin Labeling Studies of Transmembrane Signaling and Transport: Applications to Phototaxis, ABC Transporters and Symporters

Autor(en): Klare, Johann P.
Steinhoff, Heinz-Juergen 
Herausgeber: Qin, PZ
Warncke, K
Stichwörter: BACTERIORHODOPSIN MUTANTS; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Chemistry; Chemistry, Analytical; DISTANCE MEASUREMENTS; ELECTRON-PARAMAGNETIC-RESONANCE; HIGH-FIELD EPR; NITROXIDE SIDE-CHAINS; PULSE SATURATION RECOVERY; REVEALS CONFORMATIONAL EQUILIBRIA; SENSORY-RHODOPSIN-II; T4 LYSOZYME; TIME-RESOLVED DETECTION
Erscheinungsdatum: 2015
Herausgeber: ELSEVIER ACADEMIC PRESS INC
Journal: ELECTRON PARAMAGNETIC RESONANCE INVESTIGATIONS OF BIOLOGICAL SYSTEMS BY USING SPIN LABELS, SPIN PROBES, AND INTRINSIC METAL IONS, PT B
Methods in Enzymology
Volumen: 564
Startseite: 315
Seitenende: 347
Zusammenfassung: 
Membrane proteins still represent a major challenge for structural biologists. This chapter will focus on the application of continuous wave and pulsed EPR spectroscopy on spin-labeled membrane proteins. Site-directed spin labeling EPR spectroscopy has evolved as a powerful tool to study the structure and dynamics of proteins, especially membrane proteins, as this method works largely independently of the size and complexity of the biological system under investigation. This chapter describes applications of this technique to three different systems: the archaeal photoreceptor/-transducer complex SRII/HtrII as an example for transmembrane signaling and two transport systems, the histidine ATP-binding cassette transporter HisQMP, and the sodium-proline symporter PutP.
ISBN: 9780128028353
ISSN: 00766879
DOI: 10.1016/bs.mie.2015.05.025

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