CTnscr94, a conjugative transposon found in enterobacteria

Autor(en): Hochhut, B
Jahreis, K
Lengeler, JW
Schmid, K
Stichwörter: ELEMENTS; ESCHERICHIA-COLI K-12; GENE-TRANSFER; INSERTION; KLEBSIELLA-PNEUMONIAE; LACTOCOCCUS-LACTIS; Microbiology; PLASMIDS; STRAIN MG1655; SUCROSE METABOLISM; VECTORS
Erscheinungsdatum: 1997
Herausgeber: AMER SOC MICROBIOLOGY
Journal: JOURNAL OF BACTERIOLOGY
Volumen: 179
Ausgabe: 7
Startseite: 2097
Seitenende: 2102
Zusammenfassung: 
Conjugational transposons are important for horizontal gene transfer in gram-positive and gram-negative bacteria, but have not been reported yet for enteric bacteria, Salmonella senftenberg 5494-57 has previously been shown to transfer by conjugation genes for a sucrose fermentation pathway which were located on a DNA element called scr-94. We report here that the corresponding scr genes for a phosphoenolpyruvate-dependent sucrose:phosphotransferase system and a sucrose metabolic pathway are located on a large (ca. 100 kb) conjugative transposon renamed CTnscr94. The self-transmissible element integrates at two specific attachment sites in a RecA-independent way into the chromosome of Escherichia coli K-12 strains, One site was identified within pheV the structural gene for a tRNA(Phe). Sequencing of both ends of CTnscr94 revealed the presence of the 3' part of pheV on one end such that after integration of the element, a complete pheV gene is retained, CTnscr94 represents, to our knowledge, the first conjugational transposon found in enteric bacteria.
ISSN: 00219193
DOI: 10.1128/jb.179.7.2097-2102.1997

Show full item record

Page view(s)

2
Last Week
0
Last month
2
checked on Feb 27, 2024

Google ScholarTM

Check

Altmetric