CTnscr94, a conjugative transposon found in enterobacteria

DC FieldValueLanguage
dc.contributor.authorHochhut, B
dc.contributor.authorJahreis, K
dc.contributor.authorLengeler, JW
dc.contributor.authorSchmid, K
dc.date.accessioned2021-12-23T15:59:34Z-
dc.date.available2021-12-23T15:59:34Z-
dc.date.issued1997
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4004-
dc.description.abstractConjugational transposons are important for horizontal gene transfer in gram-positive and gram-negative bacteria, but have not been reported yet for enteric bacteria, Salmonella senftenberg 5494-57 has previously been shown to transfer by conjugation genes for a sucrose fermentation pathway which were located on a DNA element called scr-94. We report here that the corresponding scr genes for a phosphoenolpyruvate-dependent sucrose:phosphotransferase system and a sucrose metabolic pathway are located on a large (ca. 100 kb) conjugative transposon renamed CTnscr94. The self-transmissible element integrates at two specific attachment sites in a RecA-independent way into the chromosome of Escherichia coli K-12 strains, One site was identified within pheV the structural gene for a tRNA(Phe). Sequencing of both ends of CTnscr94 revealed the presence of the 3' part of pheV on one end such that after integration of the element, a complete pheV gene is retained, CTnscr94 represents, to our knowledge, the first conjugational transposon found in enteric bacteria.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectELEMENTS
dc.subjectESCHERICHIA-COLI K-12
dc.subjectGENE-TRANSFER
dc.subjectINSERTION
dc.subjectKLEBSIELLA-PNEUMONIAE
dc.subjectLACTOCOCCUS-LACTIS
dc.subjectMicrobiology
dc.subjectPLASMIDS
dc.subjectSTRAIN MG1655
dc.subjectSUCROSE METABOLISM
dc.subjectVECTORS
dc.titleCTnscr94, a conjugative transposon found in enterobacteria
dc.typejournal article
dc.identifier.doi10.1128/jb.179.7.2097-2102.1997
dc.identifier.isiISI:A1997WQ86300001
dc.description.volume179
dc.description.issue7
dc.description.startpage2097
dc.description.endpage2102
dc.identifier.eissn10985530
dc.publisher.place1752 N ST NW, WASHINGTON, DC 20036-2904 USA
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusGreen Published, Bronze
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