PHOTOCHEMICAL CONVERSION OF OLIGONUCLEOTIDES CONTAINING 2-CHLORO-2'-DEOXYADENOSINE - ENHANCED UV SENSITIVITY OF A MODIFIED PURINE BASE-INDUCED BY THE NEAREST-NEIGHBOR

Autor(en): RAMZAEVA, N
SEELA, F
Stichwörter: 2'-DEOXYADENOSINE; 2'-DEOXYISOGUANOSINE; 2-CHLORODEOXYADENOSINE; 5'-TRIPHOSPHATE; CELL-LINES; Chemistry; Chemistry, Medicinal; Chemistry, Multidisciplinary; DEOXYADENOSINE ANALOGS; DNA-SYNTHESIS; GLYCOSYLATION; ISOGUANOSINE; OLIGODEOXYRIBONUCLEOTIDES; Pharmacology & Pharmacy; Toxicology
Erscheinungsdatum: 1994
Herausgeber: AMER CHEMICAL SOC
Journal: CHEMICAL RESEARCH IN TOXICOLOGY
Volumen: 7
Ausgabe: 5
Startseite: 643
Seitenende: 649
Zusammenfassung: 
Irradiation of alternating dodecamers containing 2-chloro-2'-deoxyadenosine (Cl(2)dAdo) with ultraviolet light (254 nm) has been investigated. The photoconversion of Cl(2)dAdo into 2'-deoxyisoguanosine (isodGuo) and the completeness of the reaction were studied by reversed-phase HPLC and UV absorption spectra. The photosensitivity of Cl(2)dAdo within an oligonucleotide was found to be sequence-specific and depends on the nearest neighbor; 2'-deoxyguanosine residues accelerate the rate of photoconversion by a factor of 3.
ISSN: 0893228X
DOI: 10.1021/tx00041a009

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