PURIFICATION AND CHARACTERIZATION OF THE QUINATE - OXIDOREDUCTASE FROM PHASEOLUS-MUNGO SPROUTS

DC FieldValueLanguage
dc.contributor.authorKANG, XB
dc.contributor.authorSCHEIBE, R
dc.date.accessioned2021-12-23T15:59:36Z-
dc.date.available2021-12-23T15:59:36Z-
dc.date.issued1993
dc.identifier.issn00319422
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4025-
dc.description.abstractA quinate:oxidoreductase which catalyses the interconversion of (-)-quinic acid and 3-dehydroquinic acid, was extracted from the homogenate of Phaseolus mungo sprouts bought from a supermarket and purified by ammonium sulphate fractionation, anion exchange (DEAE-cellulose), hydrophobic interaction (Phenyl-Sepharose), affinity (Blue Sepharose) chromatography and gel filtration (FPLC Superose 12). Unlike most quinate:oxidoreductases of higher plants reported, which are absolutely NAD(H) specific, the enzyme from mungbean sprouts can use both NAD(H) and NADP(H) as a cofactor. Although the K(m)-values for NAD(H) and NADP(H) differ by one order of magnitude, no difference between the apparent maximum activities with NAD(H) and NADP(H) as cofactors is observed. The purified enzyme has a specific activity of about 0.5 mukat mg-1 protein (quinic to 3-dehydroquinic acid, pH 9.8, NAD) and 2.83 mukat mg -1 protein (3-dehydroquinic to quinic acid, pH 8.2, NADH). Upon gel filtration the enzyme has a M(r) of 160 000. SDS- PAGE of the peak fraction results in a single protein band of about 55 000. This result suggests that the enzyme might be a trimer.
dc.language.isoen
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofPHYTOCHEMISTRY
dc.subjectBiochemistry & Molecular Biology
dc.subjectDEHYDROQUINIC ACID
dc.subjectENZYME PURIFICATION
dc.subjectFABACEAE
dc.subjectMUNGBEAN
dc.subjectNAD+ OXIDOREDUCTASE
dc.subjectPHASEOLUS-MUNGO
dc.subjectPlant Sciences
dc.subjectPROTEIN
dc.subjectQUINATE-OXIDOREDUCTASE
dc.subjectQUINIC ACID
dc.subjectSEEDLINGS
dc.subjectSHIKIMATE PATHWAY
dc.titlePURIFICATION AND CHARACTERIZATION OF THE QUINATE - OXIDOREDUCTASE FROM PHASEOLUS-MUNGO SPROUTS
dc.typejournal article
dc.identifier.doi10.1016/0031-9422(93)85272-S
dc.identifier.isiISI:A1993LN33700006
dc.description.volume33
dc.description.issue4
dc.description.startpage769
dc.description.endpage773
dc.publisher.placeTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB
dcterms.isPartOf.abbreviationPhytochemistry
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-6140-6181-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScRe288-
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