Cloning and expression analysis of midgut chymotrypsin-like proteinases in the tobacco hornworm

DC FieldValueLanguage
dc.contributor.authorBroehan, Gunnar
dc.contributor.authorKemper, Michael
dc.contributor.authorDriemeier, Daniel
dc.contributor.authorVogelpohl, Inga
dc.contributor.authorMerzendorfer, Hans
dc.date.accessioned2021-12-23T15:59:46Z-
dc.date.available2021-12-23T15:59:46Z-
dc.date.issued2008
dc.identifier.issn00221910
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4127-
dc.description.abstractDigestion of proteins in the midgut of lepidopteran larvae relies on different trypsin and chymotrypsin isoforms. In this study we describe three chymotrypsin-like proteinases (CTLP2-4) from the larval midgut of Manduca sexta, which are closely related to CTLP1 and less closely related to another chymotrypsin (CF), two previously described proteinases present in the larval midgut of M. sexta. CTLP1-4 fit perfectly into a novel subgroup of insect CTLPs by sequence similarity and by the replacement of GP by SA in the highly conserved GDSGGP motif. When we examined CTLP expression in different tissues, most of the proteinases were predominantly expressed in the anterior and median midgut, while some were found in the Malpighian tubules. When we examined CTLP expression at different physiological states, we observed that the CTLP mRNA amounts did not differ considerably in feeding and starving larvae except for CTLP2, whose mRNA dropped significantly upon starvation. During moulting, however, the mRNA amounts of all CTLPs dropped significantly. When we immunologically examined CTLP amounts, mature proteinases were only detectable in the gut lumen of feeding and re-fed larvae, but not in that of starving or moulting larvae, suggesting that CTLP secretion is suspended during starvation or moult. (c) 2008 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB 431, GRK 612]; The authors are grateful to Dr. Helmut Wieczorek for critical comments on the manuscript. The technical help of Margret Dijvel and Ulla Madler is also gratefully acknowledged. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 431 and GRK 612).
dc.language.isoen
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartofJOURNAL OF INSECT PHYSIOLOGY
dc.subjectAEDES-AEGYPTI
dc.subjectCHITIN SYNTHASE
dc.subjectchymotrypsin-like proteinase
dc.subjectCRYSTALLINE ALPHA-CHYMOTRYPSIN
dc.subjectDIURETIC HORMONE-RECEPTOR
dc.subjectEntomology
dc.subjectMALPIGHIAN TUBULES
dc.subjectManduca sexta
dc.subjectMANDUCA-SEXTA
dc.subjectmidgut
dc.subjectPhysiology
dc.subjectSERINE PROTEASES
dc.subjectserine proteinase
dc.subjectSPODOPTERA-EXIGUA
dc.subjectSUBSTRATE-SPECIFICITY
dc.subjectV-ATPASE SUBUNIT
dc.subjectZoology
dc.titleCloning and expression analysis of midgut chymotrypsin-like proteinases in the tobacco hornworm
dc.typejournal article
dc.identifier.doi10.1016/j.jinsphys.2008.06.007
dc.identifier.isiISI:000259831000005
dc.description.volume54
dc.description.issue8
dc.description.startpage1243
dc.description.endpage1252
dc.contributor.orcid0000-0003-3766-0654
dc.contributor.researcheridH-3461-2012
dc.identifier.eissn18791611
dc.publisher.placeTHE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
dcterms.isPartOf.abbreviationJ. Insect Physiol.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidMeHa731-
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