Concanamycin a, the specific inhibitor of V-ATPases, binds to the V-o subunit c
DC Element | Wert | Sprache |
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dc.contributor.author | Huss, M | |
dc.contributor.author | Ingenhorst, G | |
dc.contributor.author | Konig, S | |
dc.contributor.author | Gassel, M | |
dc.contributor.author | Drose, S | |
dc.contributor.author | Zeeck, A | |
dc.contributor.author | Altendorf, K | |
dc.contributor.author | Wieczorek, H | |
dc.date.accessioned | 2021-12-23T15:59:48Z | - |
dc.date.available | 2021-12-23T15:59:48Z | - |
dc.date.issued | 2002 | |
dc.identifier.issn | 00219258 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/4147 | - |
dc.description.abstract | Vacuolar-type ATPase (V-ATPase) purified from the midgut of the tobacco hornworm Manduca sexta is inhibited 50% by 10 nm of the plecomacrolide concanamycin A, the specific inhibitor of V-ATPases. To determine the binding site(s) of that antibiotic in the enzyme complex, labeling with the semisynthetic 9-O-[p-(trifluoroethyldiazirinyl)-benzoyl]-21,23-dideoxy-23-[I-125]i odo-concanolide A (J-concanolide A) was performed, which still inhibits the V-ATPase 50% at a concentration of 15-20 mum. Upon treatment with UV light, a highly reactive carbene is generated from this concanamycin derivative, resulting in the formation of a covalent bond to the enzyme. In addition, the radioactive tracer 1251 makes the detection of the labeled subunit(s) feasible. Treatment of the V-1/V-o holoenzyme, the V-o complex, and the V-ATPase containing goblet cell apical membranes with concanolide resulted in the labeling of only the proteolipid, subunit c, of the proton translocating V-o complex. Binding of J-concanolide A to subunit c was prevented in a concentration-dependent manner by concanamycin A, indicating that labeling was specific. Binding was also prevented by the plecomacrolides bafilomycin A(1) and B-1 respectively, but not by the benzolactone enamide salicylihalamide, a member of a novel class of V-ATPase inhibitors. | |
dc.language.iso | en | |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | |
dc.relation.ispartof | JOURNAL OF BIOLOGICAL CHEMISTRY | |
dc.subject | BAFILOMYCIN | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | CELLS | |
dc.subject | CONTAINS | |
dc.subject | H+-ATPASE | |
dc.subject | MIDGUT | |
dc.subject | P-TYPE | |
dc.subject | PLASMA-MEMBRANE | |
dc.subject | PROTEINS | |
dc.subject | PURIFICATION | |
dc.subject | TOBACCO HORNWORM | |
dc.title | Concanamycin a, the specific inhibitor of V-ATPases, binds to the V-o subunit c | |
dc.type | journal article | |
dc.identifier.doi | 10.1074/jbc.M207345200 | |
dc.identifier.isi | ISI:000178791400051 | |
dc.description.volume | 277 | |
dc.description.issue | 43 | |
dc.description.startpage | 40544 | |
dc.description.endpage | 40548 | |
dc.contributor.orcid | 0000-0003-0672-7246 | |
dc.contributor.orcid | 0000-0002-9361-9034 | |
dc.contributor.researcherid | B-6504-2008 | |
dc.contributor.researcherid | E-4903-2010 | |
dc.identifier.eissn | 1083351X | |
dc.publisher.place | 11200 ROCKVILLE PIKE, SUITE 302, ROCKVILLE, MD, UNITED STATES | |
dcterms.isPartOf.abbreviation | J. Biol. Chem. | |
dcterms.oaStatus | hybrid | |
crisitem.author.dept | Universität Osnabrück | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.dept | FB 05 - Biologie/Chemie | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.deptid | fb05 | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.parentorg | Universität Osnabrück | - |
crisitem.author.netid | HuMa001 | - |
crisitem.author.netid | AlKa770 | - |
crisitem.author.netid | WiHe990 | - |
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geprüft am 29.05.2024