Isolation and characterization of plant N-acetyl glucosaminyltransferase I (GntI) cDNA sequences. Functional analyses in the Arabidopsis cgl mutant and in antisense plants

DC FieldValueLanguage
dc.contributor.authorWenderoth, I
dc.contributor.authorvon Schaewen, A
dc.date.accessioned2021-12-23T15:59:49Z-
dc.date.available2021-12-23T15:59:49Z-
dc.date.issued2000
dc.identifier.issn00320889
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4155-
dc.description.abstractWe report on the isolation and characterization of full-length cDNA sequences coding for N-acetylglucosaminyltransferase I (GnTI) from potato (Solanum tuberosum L.), tobacco (Nicotiana tabacum L.), and Arabidopsis. The deduced polypeptide sequences show highest homology among the solanaceous species (93% identity between potato and tobacco compared with about 75% with Arabidopsis) but share only weak homology with human GnTI (35% identity). In contrast to the corresponding enzymes from animals, all plant GnTI sequences identified are characterized by a much shorter hydrophobic membrane anchor and contain one putative N-glycosylation site that is conserved in potato and tobacco, but differs in Arabidopsis. Southern-blot analyses revealed that GntI behaves as a single-copy gene. Northern-blot analyses showed that GntI-mRNA expression is largely constitutive. Arabidopsis cgl mutants deficient in GnTI activity also possess GntI mRNA, indicating that they result from point mutations. GntI-expression constructs were tested for the ability to relieve the GnTI block in protoplasts of the Arabidopsis cgl mutant and used to obtain transgenic potato and tobacco plants that display a substantial reduction of complex glycan patterns. The latter observation indicates that production of heterologous glycoproteins with little or no antigenic glycans can be achieved in whole plants, and not in just Arabidopsis, using antisense technology.
dc.language.isoen
dc.publisherAMER SOC PLANT PHYSIOLOGISTS
dc.relation.ispartofPLANT PHYSIOLOGY
dc.subjectACETYLGLUCOSAMINYLTRANSFERASE-I
dc.subjectASPARAGINE-LINKED GLYCANS
dc.subjectCELL-WALL
dc.subjectEXPRESSION
dc.subjectGENE
dc.subjectGLYCOSYLATION
dc.subjectMOLECULAR-CLONING
dc.subjectPlant Sciences
dc.subjectPLASTIDIC GLUCOSE-6-PHOSPHATE-DEHYDROGENASE
dc.subjectPROTEINS
dc.subjectTRANSGENIC TOBACCO PLANTS
dc.titleIsolation and characterization of plant N-acetyl glucosaminyltransferase I (GntI) cDNA sequences. Functional analyses in the Arabidopsis cgl mutant and in antisense plants
dc.typejournal article
dc.identifier.doi10.1104/pp.123.3.1097
dc.identifier.isiISI:000088213300033
dc.description.volume123
dc.description.issue3
dc.description.startpage1097
dc.description.endpage1108
dc.publisher.place15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA
dcterms.isPartOf.abbreviationPlant Physiol.
dcterms.oaStatusGreen Published, Bronze
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