Structure, heterologous expression, and adhesive properties of the P-0-like myelin glycoprotein IP1 of trout CNS
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Lanwert, C | |
dc.contributor.author | Jeserich, G | |
dc.date.accessioned | 2021-12-23T16:00:16Z | - |
dc.date.available | 2021-12-23T16:00:16Z | - |
dc.date.issued | 2001 | |
dc.identifier.issn | 1059910X | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/4307 | - |
dc.description.abstract | The IP1 protein of trout CNS myelin as well as an IP1/P-0 chimeric protein were stably expressed in CHO cells. Successful targeting of the recombinant proteins to the membrane surface was verified by immunofluorescence staining. Full-length expression of IP1 could be confirmed by Western blot analysis of proteins extracted from stably transfected CHO-cells. The adhesive properties of IP1 were studied by an in vitro aggregation assay in which microscopic examination was combined with electronic particle counting. While IP1 conveyed only a weak increase in cell aggregation of transfected CHO cells, the IP1/P0 chimera was much more effective. In the presence of specific antibodies, cell aggregation was strongly reduced. The adhesive properties of P-0-like proteins are discussed considering recent crystallographic data on the atomic structure of the extracellular domain of mammalian P-0. (C) 2001 Wiley-Liss, Inc. | |
dc.language.iso | en | |
dc.publisher | WILEY-LISS | |
dc.relation.ispartof | MICROSCOPY RESEARCH AND TECHNIQUE | |
dc.subject | Anatomy & Morphology | |
dc.subject | Biology | |
dc.subject | bony fish | |
dc.subject | CELL-ADHESION | |
dc.subject | CYTOPLASMIC DOMAIN | |
dc.subject | EXTRACELLULAR DOMAIN | |
dc.subject | FISH | |
dc.subject | LEADS | |
dc.subject | Life Sciences & Biomedicine - Other Topics | |
dc.subject | membrane adhesion | |
dc.subject | Microscopy | |
dc.subject | MOLECULAR-CLONING | |
dc.subject | myelin | |
dc.subject | P-0-like protein | |
dc.subject | PERIPHERAL NERVOUS-SYSTEM | |
dc.subject | PO PROTEIN | |
dc.subject | RECOGNITION | |
dc.subject | TISSUE | |
dc.title | Structure, heterologous expression, and adhesive properties of the P-0-like myelin glycoprotein IP1 of trout CNS | |
dc.type | journal article | |
dc.identifier.doi | 10.1002/jemt.1048 | |
dc.identifier.isi | ISI:000167548400003 | |
dc.description.volume | 52 | |
dc.description.issue | 6 | |
dc.description.startpage | 637 | |
dc.description.endpage | 644 | |
dc.publisher.place | DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA | |
dcterms.isPartOf.abbreviation | Microsc. Res. Tech. |
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geprüft am 09.06.2024