RAPD-PCR investigations on sibling species of terrestrial Enchytraeus (Annelida : Oligochaeta)

Autor(en): Schirmacher, A
Schmidt, H
Westheide, W
Stichwörter: AMPLIFICATION; AMPLIFIED POLYMORPHIC DNA; ARBITRARY PRIMERS; Biochemistry & Molecular Biology; DISCRIMINATION; Ecology; Enchytraeus; Environmental Sciences & Ecology; Evolutionary Biology; GENERAL PROTEIN-PATTERNS; GENETIC-VARIATION; IDENTIFICATION; MARKERS; Oligochaeta; polymerase chain reaction (PCR); POLYMERASE CHAIN-REACTION; random amplified polymorphic DNA (RAPD); sibling species; SYSTEMATICS
Erscheinungsdatum: 1998
Herausgeber: PERGAMON-ELSEVIER SCIENCE LTD
Journal: BIOCHEMICAL SYSTEMATICS AND ECOLOGY
Volumen: 26
Ausgabe: 1
Startseite: 35
Seitenende: 44
Zusammenfassung: 
The two terrestrial enchytraeids Enchytraeus variatus and E. crypticus are true biospecies, which usually cannot be crossed in laboratory cultures. Although they are indistinguishable by light microscopy and hard to tell apart by electron microscopy (TEM of sperm), biochemically they are distinguishable at three monomorphic allozymes of 20 enzyme systems. The random amplified polymorphic DNA (RAPD)-PCR technique, employing 15 different random oligonucleotide primers, confirms an unequivocal but small genetic separation. Comparison of 199 DNA fragments ranging in length from 260 to 1800 bp revealed 15% polymorphism for E. crypticus and 19% for E. variatus. They have 79 monomorphic DNA fragments in common and are separated by 26 fragments. Cluster analyses consistently assigned single individuals to the appropriate species. The genetic distance found by RAPD analysis (Nei and Li, 1979) is only 0.17, a relatively small value for species. (C) 1998 Elsevier Science Ltd. All rights reserved.
ISSN: 03051978
DOI: 10.1016/S0305-1978(97)00079-3

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