CHARACTERIZATION OF AN AUTONOMOUSLY REPLICATING REGION FROM THE STREPTOMYCES-LIVIDANS CHROMOSOME

DC FieldValueLanguage
dc.contributor.authorZAKRZEWSKACZERWINSKA, J
dc.contributor.authorSCHREMPF, H
dc.date.accessioned2021-12-23T16:00:40Z-
dc.date.available2021-12-23T16:00:40Z-
dc.date.issued1992
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4513-
dc.description.abstractThe chromosomal replication origin of the plasmidless derivative (TK21) from Streptomyces lividans 66 has been cloned as an autonomously replicating minichromosome (pSOR1) by using the thiostrepton resistance gene as a selectable marker. pSOR1 could be recovered as a closed circular plasmid which shows high segregational instability. pSOR1 was shown to replicate in Streptomyces coelicolor A3(2) and in S. lividans 66 and hybridized with DNA from several different Streptomyces strains. Physical mapping revealed that oriC is located on a 330-kb AseI fragment of the S. coelicolor A3(2) chromosome. DNA sequence analyses showed that the cloned chromosomal oriC region contains numerous DnaA boxes which are arranged in two clusters. The preferred sequence identified in the oriC region of Escherichia coli and several other bacteria is TTATCCACA. In contrast, in S. lividans, which has a high GC content, the preferred sequence for DnaA boxes appears to be TTGTCCACA.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectCLONING
dc.subjectCONSTRUCTION
dc.subjectDNA
dc.subjectESCHERICHIA-COLI
dc.subjectINITIATION
dc.subjectMicrobiology
dc.subjectORIGIN
dc.subjectSEQUENCES
dc.titleCHARACTERIZATION OF AN AUTONOMOUSLY REPLICATING REGION FROM THE STREPTOMYCES-LIVIDANS CHROMOSOME
dc.typejournal article
dc.identifier.doi10.1128/jb.174.8.2688-2693.1992
dc.identifier.isiISI:A1992HM89900034
dc.description.volume174
dc.description.issue8
dc.description.startpage2688
dc.description.endpage2693
dc.publisher.place1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171
dcterms.isPartOf.abbreviationJ. Bacteriol.
dcterms.oaStatusGreen Published, Bronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScHi752-
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