Functional and idling rotatory motion within F-1-ATPase

DC FieldValueLanguage
dc.contributor.authorSabbert, D
dc.contributor.authorEngelbrecht, S
dc.contributor.authorJunge, W
dc.date.accessioned2021-12-23T16:00:54Z-
dc.date.available2021-12-23T16:00:54Z-
dc.date.issued1997
dc.identifier.issn00278424
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4647-
dc.description.abstractATP synthase mediates proton flow through its membrane portion, F-0, which drives the synthesis of ATP in its headpiece, F-1. The F-1-portion contains a hexagonal array of three subunits alpha and three beta encircling a central subunit gamma, that in turn interacts with a smaller epsilon and with F-0. Recently we reported that the application of polarized absorption recovery after photobleaching showed the ATP-driven rotation of gamma over at least two, if not three, beta. Here we extend probes of such rotation aided by a new theory for assessing continuous versus stepped, Brownian versus unidirectional molecular motion. The observed relaxation of the absorption anisotropy is fully compatible with a unidirectional and stepping rotation of gamma over three equidistantly spaced angular positions in the hexagon formed by the alternating subunits alpha and beta. The results strongly support a rotational catalysis with equal participation of all three catalytic sites. In addition we report a limited rotation of gamma without added nucleotides, perhaps idling and of Brunnian nature, that covers only a narrow angular domain.
dc.language.isoen
dc.publisherNATL ACAD SCIENCES
dc.relation.ispartofPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
dc.subjectCHLOROPLAST ATP SYNTHASE
dc.subjectCOUPLING FACTOR-I
dc.subjectDELTA-SUBUNIT
dc.subjectESCHERICHIA-COLI F1-ATPASE
dc.subjectMultidisciplinary Sciences
dc.subjectMUTANT STRAINS
dc.subjectNUCLEOTIDE-BINDING
dc.subjectOXIDATIVE-PHOSPHORYLATION
dc.subjectPROPIONIGENIUM-MODESTUM
dc.subjectScience & Technology - Other Topics
dc.subjectSITE MECHANISM
dc.subjectTHYLAKOID MEMBRANES
dc.titleFunctional and idling rotatory motion within F-1-ATPase
dc.typejournal article
dc.identifier.doi10.1073/pnas.94.9.4401
dc.identifier.isiISI:A1997WX36200031
dc.description.volume94
dc.description.issue9
dc.description.startpage4401
dc.description.endpage4405
dc.publisher.place2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418
dcterms.isPartOf.abbreviationProc. Natl. Acad. Sci. U. S. A.
dcterms.oaStatusGreen Published, Bronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidJuWo587-
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