The small GTPase KIRho5 responds to oxidative stress and affects cytokinesis

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dc.contributor.authorMusielak, Marius
dc.contributor.authorSterk, Carolin C.
dc.contributor.authorSchubert, Felix
dc.contributor.authorMeyer, Christian
dc.contributor.authorPaululat, Achim
dc.contributor.authorHeinisch, Juergen J.
dc.date.accessioned2021-12-23T16:01:00Z-
dc.date.available2021-12-23T16:01:00Z-
dc.date.issued2021
dc.identifier.issn00219533
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4717-
dc.description.abstractRho5 is the yeast homolog of the human small GTPase Rac1. We characterized the genes encoding Rho5 and the subunits of its dimeric activating guanine-nucleotide-exchange factor (GEF), Dck1 and Lmo1 , in the yeast Kluyveromyces twits. Rapid translocation of the three GFP-tagged components to mitochondria upon oxidative stress and carbon starvation indicate a similar function of KlRho5 in energy metabolism and mitochondrial dynamics as described for its Saccharomyces cerevisiae homolog. Accordingly, Klrho5 deletion mutants are hyper-resistant towards hydrogen peroxide. Moreover, synthetic lethalities of rho5 deletions with key components in nutrient sensing, such as sch9 and gpr1, are not conserved in K. lactis. Instead, Klrho5 deletion mutants display morphological defects with strengthened lateral cell walls and protruding bud scars. The latter result from aberrant cytokinesis, as observed by following the budding process in vivo and by transmission electron microscopy of the bud neck region. This phenotype can be suppressed by K/CDC42(G)(12v), which encodes a hyper-active variant. Data from livecell fluorescence microscopy support the notion that KIRho5 interferes with the actin moiety of the contractile actomyosin ring, with consequences different from those previously reported for mutants lacking myosin.
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [HE 1880/6-1]; This work was funded by a grant from the Deutsche Forschungsgemeinschaft (DFG; HE 1880/6-1).
dc.language.isoen
dc.publisherCOMPANY BIOLOGISTS LTD
dc.relation.ispartofJOURNAL OF CELL SCIENCE
dc.subjectBUDDING YEAST
dc.subjectCell Biology
dc.subjectCell wall
dc.subjectGENETICS
dc.subjectMECHANISM
dc.subjectMETABOLISM
dc.subjectMitochondrion
dc.subjectNutrient stress
dc.subjectPROTEIN
dc.subjectRho-type GTPase
dc.subjectRHO5P
dc.subjectRING
dc.subjectTRANSCRIPTION
dc.subjectVERSATILE
dc.subjectYeast
dc.titleThe small GTPase KIRho5 responds to oxidative stress and affects cytokinesis
dc.typejournal article
dc.identifier.doi10.1242/jcs.258301
dc.identifier.isiISI:000704598000009
dc.description.volume134
dc.description.issue18
dc.contributor.orcid0000-0002-8845-6859
dc.contributor.orcid0000-0001-8303-5636
dc.identifier.eissn14779137
dc.publisher.placeBIDDER BUILDING, STATION RD, HISTON, CAMBRIDGE CB24 9LF, ENGLAND
dcterms.isPartOf.abbreviationJ. Cell Sci.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-8845-6859-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidPaAc947-
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