Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation
DC Element | Wert | Sprache |
---|---|---|
dc.contributor.author | Gauthier, Anne | |
dc.contributor.author | Brandt, Roland | |
dc.date.accessioned | 2021-12-23T16:01:05Z | - |
dc.date.available | 2021-12-23T16:01:05Z | - |
dc.date.issued | 2010 | |
dc.identifier.issn | 14316730 | |
dc.identifier.uri | https://osnascholar.ub.uni-osnabrueck.de/handle/unios/4756 | - |
dc.description.abstract | Alzheimer's disease and other neurodegenerative disorders share some common features at the cellular level, which are often associated with a change in cytoskeletal dynamics. Live cell imaging has been applied to study various aspects of cell physiology including cytoskeletal dynamics. Recently, fluorescence photoactivation (FPA) has been developed as a novel approach to analyze protein dynamics in living cells with high sensitivity. Here, we describe the application of HA to determine interactions and mobility of the microtubule-associated protein tau in neurons as an example for a disease-related cytoskeletal protein and discuss further applications of FPA to study cytoskeletal dynamics in neurodegenerative diseases. | |
dc.description.sponsorship | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG); This work was supported by a fellowship of the graduate college 612 of the Deutsche Forschungsgemeinschaft (A.G.). We also thank Dr. Lidia Bakota for helpful suggestions on the manuscript. | |
dc.language.iso | en | |
dc.publisher | WALTER DE GRUYTER GMBH | |
dc.relation.ispartof | BIOLOGICAL CHEMISTRY | |
dc.subject | Alzheimer's disease | |
dc.subject | Biochemistry & Molecular Biology | |
dc.subject | DEGENERATION | |
dc.subject | EXPRESSION | |
dc.subject | microtubule-associated protein | |
dc.subject | neurodegeneration | |
dc.subject | photoactivatable GFP | |
dc.subject | photoactivation | |
dc.subject | PROTEINS | |
dc.subject | SCLEROSIS | |
dc.subject | TAU | |
dc.subject | tau protein | |
dc.subject | TRANSPORT | |
dc.title | Live cell imaging of cytoskeletal dynamics in neurons using fluorescence photoactivation | |
dc.type | review | |
dc.identifier.doi | 10.1515/BC.2010.060 | |
dc.identifier.isi | ISI:000278486500007 | |
dc.description.volume | 391 | |
dc.description.issue | 6 | |
dc.description.startpage | 639 | |
dc.description.endpage | 643 | |
dc.contributor.orcid | 0000-0003-0101-1257 | |
dc.identifier.eissn | 14374315 | |
dc.publisher.place | GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY | |
dcterms.isPartOf.abbreviation | Biol. Chem. | |
crisitem.author.orcid | 0000-0003-0101-1257 | - |
crisitem.author.netid | BrRo587 | - |
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