Synthesis of the Streptomyces lividans maltodextrin ABC transporter depends on the presence of the regulator MalR

DC FieldValueLanguage
dc.contributor.authorSchlosser, A
dc.contributor.authorWeber, A
dc.contributor.authorSchrempf, H
dc.date.accessioned2021-12-23T16:01:09Z-
dc.date.available2021-12-23T16:01:09Z-
dc.date.issued2001
dc.identifier.issn03781097
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4788-
dc.description.abstractDuring growth with maltotriose or amylose, Streptomyces lividans and Streptomyces coelicolor A3(2) synthesize a maltodextrin uptake system with highest specificity for maltotriose. The transport activity is absent in mutants of S. coelicolor A3(2) lacking a functional MalE binding protein. Cloning and sequencing data suggest that the mal operon of S. coelicolor A3(2) corresponds to the one of S. lividans and that the deduced S. lividans Reg1 amino acid sequence is identical to that of MalR from S. coelicolor A3(2). It can be concluded that both strains have the same ABC transport system for maltodextrins. The S. lividans malR was cloned in Escherichia coli in frame with six histidine-encoding codons. The resulting, purified 6HisMalR(Sl) was shown to bind to two motifs within the S. lividans malR-malE intergenic region and to dissociate in the presence of maltopentaose. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofFEMS MICROBIOLOGY LETTERS
dc.subjectABC transporter
dc.subjectCELLOBIOSE
dc.subjectCOELICOLOR A3(2)
dc.subjectCRYSTAL-STRUCTURE
dc.subjectESCHERICHIA-COLI
dc.subjectGENE
dc.subjectglucose repression
dc.subjectLACI-GALR FAMILY
dc.subjectMalR repressor
dc.subjectMALTOSE-BINDING
dc.subjectmaltose/maltodextrin
dc.subjectMEMBER
dc.subjectMicrobiology
dc.subjectPROTEIN
dc.subjectReg1 repressor
dc.subjectregulation of transcription
dc.subjectSTREPTOCOCCUS-PNEUMONIAE
dc.subjectStreptomyces
dc.titleSynthesis of the Streptomyces lividans maltodextrin ABC transporter depends on the presence of the regulator MalR
dc.typejournal article
dc.identifier.doi10.1111/j.1574-6968.2001.tb10544.x
dc.identifier.isiISI:000167599500014
dc.description.volume196
dc.description.issue1
dc.description.startpage77
dc.description.endpage83
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationFEMS Microbiol. Lett.
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScHi752-
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