VISUALIZATION OF ALPHA-CHITIN WITH A SPECIFIC CHITIN-BINDING PROTEIN (CHB1) FROM STREPTOMYCES-OLIVACEOVIRIDIS
Autor(en): | ZELTINS, A SCHREMPF, H |
Stichwörter: | Biochemical Research Methods; Biochemistry & Molecular Biology; CELL-WALL; Chemistry; Chemistry, Analytical; GENE; SACCHAROMYCES-CEREVISIAE; WALL SYNTHESIS | Erscheinungsdatum: | 1995 | Herausgeber: | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Enthalten in: | ANALYTICAL BIOCHEMISTRY | Band: | 231 | Ausgabe: | 2 | Startseite: | 287 | Seitenende: | 294 | Zusammenfassung: | Recently we identified a so far unique protein (CHB1) which interacts specifically with crystalline alpha-chitin. Having optimized the binding conditions for CHB1 coupled with fluorescein isothiocyanate (FITC), we succeeded in developing a highly sensitive assay to detect alpha-chitin, CHB1-FITC interacted neither with beta- or colloidal chitin nor with chitooligomers or cellulose. With the help of fluorescence or confocal laser microscopy, the relative location of crystalline alpha-chitin within various native samples of fungi and other organisms can be clearly and rapidly visualized. (C) 1995 Academic Press, Inc. |
ISSN: | 00032697 | DOI: | 10.1006/abio.1995.0053 |
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geprüft am 06.06.2024