SUBCLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF TRKG, A GENE THAT ENCODES AN INTEGRAL MEMBRANE-PROTEIN INVOLVED IN POTASSIUM UPTAKE VIA THE TRK SYSTEM OF ESCHERICHIA-COLI

DC FieldValueLanguage
dc.contributor.authorSCHLOSSER, A
dc.contributor.authorKLUTTIG, S
dc.contributor.authorHAMANN, A
dc.contributor.authorBAKKER, EP
dc.date.accessioned2021-12-23T16:01:14Z-
dc.date.available2021-12-23T16:01:14Z-
dc.date.issued1991
dc.identifier.issn00219193
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4834-
dc.description.abstractThe trkG gene encodes a component of the K+ uptake system Trk and is located at 30.5 min inside the lambdoid prophage region rac of the Escherichia coli chromosome. trkG was subcloned, its nucleotide sequence was determined, and its product was identified in a minicell system. The open reading frame of 1,455 bp encodes a hydrophobic membrane protein with a calculated molecular weight of 53,493 that is predicted to contain up to 12 transmembrane helices. The trkG gene product behaved as a hydrophobic membrane protein; it was found exclusively in the membrane fraction of the minicells and its migration in sodium dodecyl sulfate-polyacrylamide gel electrophoresis was anomalous, indicating an apparent molecular weight of 35,000. The trkG gene contains an exceptionally high proportion of infrequently used codons, raising the question of the origin of this gene. trkG does not appear to be a prophage gene since no similarity was observed between the nucleotide sequence of trkG or the amino acid sequence of its product and the sequences of genes or proteins from bacteriophage lambda.
dc.language.isoen
dc.publisherAMER SOC MICROBIOLOGY
dc.relation.ispartofJOURNAL OF BACTERIOLOGY
dc.subjectCATION-TRANSPORT
dc.subjectK+-TRANSPORT
dc.subjectK-12
dc.subjectLOCUS
dc.subjectMAP
dc.subjectMicrobiology
dc.subjectPLOT
dc.subjectPROLINE CARRIER
dc.titleSUBCLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF TRKG, A GENE THAT ENCODES AN INTEGRAL MEMBRANE-PROTEIN INVOLVED IN POTASSIUM UPTAKE VIA THE TRK SYSTEM OF ESCHERICHIA-COLI
dc.typejournal article
dc.identifier.isiISI:A1991FL18100017
dc.description.volume173
dc.description.issue10
dc.description.startpage3170
dc.description.endpage3176
dc.publisher.place1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171
dcterms.isPartOf.abbreviationJ. Bacteriol.
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