Molecular cloning and functional characterization of Shaw-related potassium channels of trout CNS

Autor(en): Panofen, F
Rabe, H
Henne, J
Jeserich, G
Stichwörter: bony fish; CELLS; CENTRAL-NERVOUS-SYSTEM; developmental expression; DIFFERENTIAL EXPRESSION; DIVERSITY; electrophysiology; GATED K+ CHANNELS; GENE; KV3.1; molecular structure; NEURONS; Neurosciences; Neurosciences & Neurology; PHARMACOLOGICAL CHARACTERIZATION; proteinkinase C; RAT-BRAIN; Shaw-type channel
Erscheinungsdatum: 2000
Herausgeber: ELSEVIER SCIENCE BV
Journal: MOLECULAR BRAIN RESEARCH
Volumen: 83
Ausgabe: 1-2
Startseite: 9
Seitenende: 19
Zusammenfassung: 
Two Shaw-related potassium channels expressed in the CNS of trout were PCR cloned and sequenced. Trawl was identified as a fish homologue to mammalian K(v)3.1, while Traw2 could not be exactly classified. Upon heterologous expression Traw1 exhibited a high threshold (-20 mV) non-inactivating delayed rectifier current that was efficiently blocked by submicromolar concentrations of TEA, 4-AP and quinine but not by alpha -DTX or apamin. The amplitude of the Trawl induced current was reduced by the phorbol ester TPA, the effect being prevented by the proteinkinase inhibitor H7. Transcripts of both Shaw- related channels possess a widespread distribution in the mature brain tissue and outside the nervous system are detectable in muscle but not in liver. During brain development Trawl mRNA was initially identified at stage 31 (shortly after hatching) while transcripts encoding Traw2 were detectable already at stage 28 (1 week before hatching). (C) 2000 Elsevier Science B.V. All rights reserved.
ISSN: 0169328X
DOI: 10.1016/S0169-328X(00)00159-5

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