Molecular cloning and functional characterization of Shaw-related potassium channels of trout CNS

DC FieldValueLanguage
dc.contributor.authorPanofen, F
dc.contributor.authorRabe, H
dc.contributor.authorHenne, J
dc.contributor.authorJeserich, G
dc.date.accessioned2021-12-23T16:01:25Z-
dc.date.available2021-12-23T16:01:25Z-
dc.date.issued2000
dc.identifier.issn0169328X
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4951-
dc.description.abstractTwo Shaw-related potassium channels expressed in the CNS of trout were PCR cloned and sequenced. Trawl was identified as a fish homologue to mammalian K(v)3.1, while Traw2 could not be exactly classified. Upon heterologous expression Traw1 exhibited a high threshold (-20 mV) non-inactivating delayed rectifier current that was efficiently blocked by submicromolar concentrations of TEA, 4-AP and quinine but not by alpha -DTX or apamin. The amplitude of the Trawl induced current was reduced by the phorbol ester TPA, the effect being prevented by the proteinkinase inhibitor H7. Transcripts of both Shaw- related channels possess a widespread distribution in the mature brain tissue and outside the nervous system are detectable in muscle but not in liver. During brain development Trawl mRNA was initially identified at stage 31 (shortly after hatching) while transcripts encoding Traw2 were detectable already at stage 28 (1 week before hatching). (C) 2000 Elsevier Science B.V. All rights reserved.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofMOLECULAR BRAIN RESEARCH
dc.subjectbony fish
dc.subjectCELLS
dc.subjectCENTRAL-NERVOUS-SYSTEM
dc.subjectdevelopmental expression
dc.subjectDIFFERENTIAL EXPRESSION
dc.subjectDIVERSITY
dc.subjectelectrophysiology
dc.subjectGATED K+ CHANNELS
dc.subjectGENE
dc.subjectKV3.1
dc.subjectmolecular structure
dc.subjectNEURONS
dc.subjectNeurosciences
dc.subjectNeurosciences & Neurology
dc.subjectPHARMACOLOGICAL CHARACTERIZATION
dc.subjectproteinkinase C
dc.subjectRAT-BRAIN
dc.subjectShaw-type channel
dc.titleMolecular cloning and functional characterization of Shaw-related potassium channels of trout CNS
dc.typejournal article
dc.identifier.doi10.1016/S0169-328X(00)00159-5
dc.identifier.isiISI:000165342400002
dc.description.volume83
dc.description.issue1-2
dc.description.startpage9
dc.description.endpage19
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationMol. Brain Res.
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