Partial characterization of the 5'-flanking region of trout IP: A Po-like gene containing a PLP-like promoter

DC FieldValueLanguage
dc.contributor.authorJeserich, G
dc.contributor.authorStrelau, J
dc.contributor.authorLanwert, C
dc.date.accessioned2021-12-23T16:01:26Z-
dc.date.available2021-12-23T16:01:26Z-
dc.date.issued1997
dc.identifier.issn03604012
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/4962-
dc.description.abstractThe IP gene of trout encodes two Po-like glycoproteins which are expressed by oligodendrocytes in the fish CNS, A 679 bp fragment of its 5'-flanking region was isolated from a genomic library and sequenced. The transcription start point was determined 124 bp upstream the ATG initiator codon by primer extension analysis, Apart from a modified TATA-box and an inverted CCAAT-box located at canonical distances from the transcription start site several eucaryotic cis-acting regulatory elements were identified in the 679 bp upstream region, including an AP-1 binding site, a brain specific Sp1 motif, a cyclic AMP responsive element and a consensus sequence for POU homeodomain protein binding, The occurence of respective DNA-binding proteins for Sp1, AP-1 and POU in the nuclei of trout oligodendrocyte progenitor cells was verified by gel retardation experiments, Functional activity of various subfragments of the 679 bp upstream region was demonstrated by CAT reporter gene analysis, A computer-assisted sequence alignment of the trout IP 5'-flanking end with the corresponding region of the mammalian PLP gene promoter revealed four sites of high homology, while similarity with the mammalian Po gene promotor was low. The results are discussed with respect to the phylogenetic shift from Po-like proteins to PLP during evolution of the vertebrate CNS myelin sheath. (C) 1997 Wiley-Liss, Inc.
dc.language.isoen
dc.publisherWILEY-LISS
dc.relation.ispartofJOURNAL OF NEUROSCIENCE RESEARCH
dc.subjectBRAIN
dc.subjectCELLS
dc.subjectCLONING
dc.subjectCNS MYELIN
dc.subjectevolution
dc.subjectFISH MYELIN
dc.subjectGLYCOPROTEINS
dc.subjectMAJOR STRUCTURAL PROTEIN
dc.subjectNeurosciences
dc.subjectNeurosciences & Neurology
dc.subjectPERIPHERAL MYELIN
dc.subjectPo-like protein
dc.subjectpromoter region
dc.subjectproteolipidprotein
dc.subjectSEQUENCE
dc.subjectTRANSCRIPTION
dc.titlePartial characterization of the 5'-flanking region of trout IP: A Po-like gene containing a PLP-like promoter
dc.typejournal article
dc.identifier.doi10.1002/(SICI)1097-4547(19971201)50:5<781::AID-JNR14>3.0.CO;2-4
dc.identifier.isiISI:A1997YH74800013
dc.description.volume50
dc.description.issue5
dc.description.startpage781
dc.description.endpage790
dc.publisher.placeDIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012
dcterms.isPartOf.abbreviationJ. Neurosci. Res.
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