Diffusion and Interaction Dynamics of Individual Membrane Protein Complexes Confined in Micropatterned Polymer-Supported Membranes

Autor(en): Waichman, Sharon
Roder, Friedrich
Richter, Christian P.
Birkholz, Oliver
Piehler, Jacob 
Stichwörter: BILAYERS; CELL-MEMBRANES; Chemistry; Chemistry, Multidisciplinary; Chemistry, Physical; GIANT UNILAMELLAR VESICLES; IMMOBILIZATION; LATERAL DIFFUSION; LIPID-MEMBRANES; Materials Science; Materials Science, Multidisciplinary; membrane proteins; MICROSCOPY; Nanoscience & Nanotechnology; photolithography; Physics; Physics, Applied; Physics, Condensed Matter; polymer-supported membranes; proteinprotein interactions; RECEPTOR INTERACTIONS; Science & Technology - Other Topics; SINGLE-MOLECULE FLUORESCENCE; single-molecule imaging; TRACKING
Erscheinungsdatum: 2013
Herausgeber: WILEY-V C H VERLAG GMBH
Journal: SMALL
Volumen: 9
Ausgabe: 4
Startseite: 570
Seitenende: 577
Zusammenfassung: 
Micropatterned polymer-supported membranes (PSM) are established as a tool for confining the diffusion of transmembrane proteins for single molecule studies. To this end, a photochemical surface modification with hydrophobic tethers on a PEG polymer brush is implemented for capturing of lipid vesicles and subsequent fusion. Formation of contiguous membranes within micropatterns is confirmed by scanning force microscopy, fluorescence recovery after photobleaching (FRAP), and super-resolved single-molecule tracking and localization microscopy. Free diffusion of transmembrane proteins reconstituted into micropatterned PSM is demonstrated by FRAP and by single-molecule tracking. By exploiting the confinement of diffusion within micropatterned PSM, the diffusion and interaction dynamics of individual transmembrane receptors are quantitatively resolved.
ISSN: 16136810
DOI: 10.1002/smll.201201530

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