Transcriptional regulation of NADP-dependent malate dehydrogenase: Comparative genetics and identification of DNA-binding proteins

DC FieldValueLanguage
dc.contributor.authorHameister, Steffen
dc.contributor.authorBecker, Beril
dc.contributor.authorHoltgrefe, Simone
dc.contributor.authorStrodtkoetter, Inga
dc.contributor.authorLinke, Vera
dc.contributor.authorBackhausen, Jan E.
dc.contributor.authorScheibe, Renate
dc.date.accessioned2021-12-23T16:01:38Z-
dc.date.available2021-12-23T16:01:38Z-
dc.date.issued2007
dc.identifier.issn00222844
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5075-
dc.description.abstractThe transcriptional regulation of NADP-malate dehydrogenase (NADP-MDH) was analyzed in Arabidopsis ecotypes and other Brassicaceae. The amount of transcript increased twofold after transfer into low temperature (12 degrees C) or high light (750 mu E) in all species. Analysis of the genomic DNA reveals that the NADP-MDH gene (At5g58330 in A. thaliana) in Brassicaceae is located between two other genes (At5g58320 and At5g58340 in Arabidopsis), both encoded on the opposite DNA strand. No promoter elements were identified in 5 ` direction of the NADP-MDH gene, and the expression of NADP-MDH was not affected in knock-out plants carrying a DNA insert in the 5 ` region. A yeast-one hybrid approach yielded only three DNA-binding proteins for the 500-bp fragment located upstream of the ATG sequence, but 34 proteins for its coding region. However, in Chlamydomonas and in some Poaceae, which do not possess any genes within the 1200 bp upstream region, typical promoter elements were identified. Alignments of genomic DNA reveal that, in contrast to Poaceae, the introns are highly conserved within Brassicaceae. We conclude that in Brassicaceae the majority of regulatory elements are located within the coding region. The NADP-MDH gene of both families evolved from a common precursor, similar to the gene in Chlamydomonas. Changes in the selection pressure allowed the insertion of At5g58340 into the promoter region of a common ancestor. When the demand for transcriptional regulation increased, At5g58340 disappeared in Poaceae, and a promoter developed in the 5 ` region. In contrast, Brassicaceae maintained At5g58340 and shifted all regulatory elements into the coding region of NADP-MDH.
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofJOURNAL OF MOLECULAR EVOLUTION
dc.subjectACCLIMATION
dc.subjectArabidopsis
dc.subjectARABIDOPSIS-THALIANA
dc.subjectBiochemistry & Molecular Biology
dc.subjectBrassicaceae
dc.subjectevolution
dc.subjectEvolutionary Biology
dc.subjectEXPRESSION
dc.subjectgene organization
dc.subjectGENES
dc.subjectGenetics & Heredity
dc.subjectGENOME ANALYSIS
dc.subjectgenomic DNA
dc.subjectknock-out
dc.subjectLIGHT ACTIVATION
dc.subjectORGANIZATION
dc.subjectPHOTOSYNTHESIS
dc.subjectPoaceae
dc.subjectSTRESS RESPONSES
dc.subjectTOBACCO PLANTS
dc.subjectyeast-one hybrid
dc.titleTranscriptional regulation of NADP-dependent malate dehydrogenase: Comparative genetics and identification of DNA-binding proteins
dc.typejournal article
dc.identifier.doi10.1007/s00239-007-9025-9
dc.identifier.isiISI:000250626400008
dc.description.volume65
dc.description.issue4
dc.description.startpage437
dc.description.endpage455
dc.identifier.eissn14321432
dc.publisher.placeONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES
dcterms.isPartOf.abbreviationJ. Mol. Evol.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-6140-6181-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScRe288-
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