7-deaza-2 `-deoxyinosine: A stable nucleoside with the ambiguous base pairing properties of 2 `-deoxyinosine

Autor(en): Seela, F
Mittelbach, C
Stichwörter: 2'-DEOXYRIBONUCLEOSIDES; 5-NITROINDOLE; Biochemistry & Molecular Biology; DEOXYINOSINE; DNA; HYBRIDIZATION; IDENTIFICATION; NUCLEOBASE; OLIGONUCLEOTIDES; PHASE-TRANSFER GLYCOSYLATION; STABILITIES
Erscheinungsdatum: 1999
Herausgeber: MARCEL DEKKER INC
Journal: NUCLEOSIDES & NUCLEOTIDES
Volumen: 18
Ausgabe: 3
Startseite: 425
Seitenende: 441
Zusammenfassung: 
The base pairing ambiguity of 7-deaza-2'-deoxyinosine (c(7)Id, 2) was studied and was found to be the same as that of 2'-deoxyinosine. The duplex stability decreases in the order [d(c(7)I-C) > d(c(7)I-A) > d(c(7)I-T) > d(c(7)I-G)]. Modified nucleosides were used to probe the various base pair motifs which were the same for dl and c(7)I(d). The 7-deazapurine nucleoside (2) is extremely stable against acid or base. As oligonucleotides can be prepared using phosphoramidite chemistry and DNA is accessible by enzymic polymerisation of the triphosphate of 2, the latter can be used as an universal nucleoside for the sequencing of DNA by chemical degradation and is otherwise a facile substitute of 2'-deoxyinosine when stability in acidic or alkaline solution is required.
ISSN: 07328311
DOI: 10.1080/15257779908043087

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