ION CHANNELS IN THE THYLAKOID MEMBRANE (A PATCH-CLAMP STUDY)

DC FieldValueLanguage
dc.contributor.authorENZ, C
dc.contributor.authorSTEINKAMP, T
dc.contributor.authorWAGNER, R
dc.date.accessioned2021-12-23T16:01:45Z-
dc.date.available2021-12-23T16:01:45Z-
dc.date.issued1993
dc.identifier.issn00063002
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5145-
dc.description.abstractThylakoid membranes were fused with azolectin liposomes and enlarged to giant thylakoid/liposome vesicles with diameters between 20 mum and 50 mum by a modified dehydration/rehydration procedure. Ion transport across the membrane of these giant thylakoid/liposome vesicles was investigated by the patch-clamp technique. Single-channel currents were observed and four different specific ion conductances could be distinguished. Our results indicate that these conductances can be attributed to three types of ion channel: a putative potassium channel with a conductance of 110 pS (in 100 mM KCl) a chloride channel with a conductance of 220 pS (in 100 mM KCl) and a moderate selectivity of about 4 : 1 for anions over cations. The third type of putative ion channel we observed was permeable to Mg2+ and Ca2+ ions with conductances of 20 pS for Ca2+ and 35 pS for Mg2+, respectively.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofBIOCHIMICA ET BIOPHYSICA ACTA
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCHLOROPLAST
dc.subjectCO2 FIXATION
dc.subjectELECTRON TRANSPORT
dc.subjectINTACT CHLOROPLASTS
dc.subjectION CHANNEL
dc.subjectLIGHT-DEPENDENT CHANGES
dc.subjectMEMBRANE POTENTIAL
dc.subjectPHOTOPHOSPHORYLATION
dc.subjectSTROMAL MG2+ CONCENTRATION
dc.subjectTHYLAKOID MEMBRANE
dc.titleION CHANNELS IN THE THYLAKOID MEMBRANE (A PATCH-CLAMP STUDY)
dc.typejournal article
dc.identifier.doi10.1016/0005-2728(93)90217-4
dc.identifier.isiISI:A1993LG44100008
dc.description.volume1143
dc.description.issue1
dc.description.startpage67
dc.description.endpage76
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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