A routine method of DNA-extraction from extremely small metazoans, e.g. single rotifer specimens for RAPD-PCR analyses

Autor(en): Leutbecher, C
Stichwörter: AMPLIFIED POLYMORPHIC DNA; BOOTSTRAP; BRACHIONUS-PLICATILIS; CHAIN-REACTION PCR; CONFIDENCE; DNA-isolation; IDENTIFICATION; Marine & Freshwater Biology; PATTERNS; PHYLOGENY; PRIMERS; RAPD-PCR; rotifer; single specimen analysis; SYSTEMATICS
Erscheinungsdatum: 2000
Herausgeber: SPRINGER
Journal: HYDROBIOLOGIA
Volumen: 437
Ausgabe: 1-3
Startseite: 133
Seitenende: 137
Zusammenfassung: 
A simple and efficient method has been developed for isolating genomic DNA from single specimens of extremely small metazoan animals, e.g. rotifers (Rotifera: Monogononta). The amount and quality of the extracted DNA allows the Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) to be employed with up to three random primers per specimen. Reproducibility of RAPD-fingerprints has been checked and verified. Exemplarily, RAPD patterns of three Brachionus species (B. angularis, B. calyciflorus, B. urceolaris) amplified with the primer OPA 07 are demonstrated.
ISSN: 00188158
DOI: 10.1023/A:1026546705582

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