Spin labeling EPR
| Autor(en): | Klare, Johann P. Steinhoff, Heinz-Juergen |
Stichwörter: | ALPHA-AMINO-ACIDS; DEER; DISTANCE MEASUREMENTS; ELECTRON-PARAMAGNETIC-RESONANCE; HIGH-FIELD EPR; MOLECULAR-DYNAMICS SIMULATIONS; Nitroxide; NITROXIDE SIDE-CHAINS; PELDOR; Photosynthetic reaction center; Plant Sciences; Polarity; SATURATION RECOVERY EPR; Sensory rhodopsin; SENSORY-RHODOPSIN-II; SITE-SPECIFIC INCORPORATION; Spin label accessibility; Spin label mobility; TIME-RESOLVED DETECTION | Erscheinungsdatum: | 2009 | Herausgeber: | SPRINGER | Journal: | PHOTOSYNTHESIS RESEARCH | Volumen: | 102 | Ausgabe: | 2-3, SI | Startseite: | 377 | Seitenende: | 390 | Zusammenfassung: | Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling. Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the sensory rhodopsin-transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis and the photosynthetic reaction center from Rhodobacter sphaeroides R26. |
ISSN: | 01668595 | DOI: | 10.1007/s11120-009-9490-7 |
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