Spin labeling EPR

Autor(en): Klare, Johann P.
Steinhoff, Heinz-Juergen 
Stichwörter: ALPHA-AMINO-ACIDS; DEER; DISTANCE MEASUREMENTS; ELECTRON-PARAMAGNETIC-RESONANCE; HIGH-FIELD EPR; MOLECULAR-DYNAMICS SIMULATIONS; Nitroxide; NITROXIDE SIDE-CHAINS; PELDOR; Photosynthetic reaction center; Plant Sciences; Polarity; SATURATION RECOVERY EPR; Sensory rhodopsin; SENSORY-RHODOPSIN-II; SITE-SPECIFIC INCORPORATION; Spin label accessibility; Spin label mobility; TIME-RESOLVED DETECTION
Erscheinungsdatum: 2009
Herausgeber: SPRINGER
Journal: PHOTOSYNTHESIS RESEARCH
Volumen: 102
Ausgabe: 2-3, SI
Startseite: 377
Seitenende: 390
Zusammenfassung: 
Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling. Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the sensory rhodopsin-transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis and the photosynthetic reaction center from Rhodobacter sphaeroides R26.
ISSN: 01668595
DOI: 10.1007/s11120-009-9490-7

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