The Methanocaldococcus jannaschii protein Mj0968 is not a P-type ATPase

DC FieldValueLanguage
dc.contributor.authorBramkamp, M
dc.contributor.authorGassel, M
dc.contributor.authorHerkenhoff-Hesselmann, B
dc.contributor.authorBertrand, J
dc.contributor.authorAltendorf, K
dc.date.accessioned2021-12-23T16:02:27Z-
dc.date.available2021-12-23T16:02:27Z-
dc.date.issued2003
dc.identifier.issn18733468
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5422-
dc.description.abstractThe Methanocaldococcus jannaschii (formerly Methanococcus jannaschii) protein Mj0968 has been reported to represent a soluble P-type ATPase [Ogawa et al., FEBS Lett. 471 (2000) 99-102]. In this study, we report that the heterologously expressed Mj0968-His(10) protein exhibits high rates of phosphatase activity, whereas only very low ATPase activity was measured. Replacement of the aspartate residue in the DSAGT motif (D7A), which becomes phosphorylated during the reaction cycle of P-type ATPases, does not affect the V-max, but only the K-M of the reaction. Labeling studies with [gamma-P-32]ATP and [alpha-P-32]ATP revealed that the previously reported labeling experiments [Ogawa et al., 2000] do not necessarily show phosphorylation of Mj0968, but rather point to ATP binding. Binding studies with trinitrophenyl adenosine nucleotides showed low apparent K-d values for those molecules. These results provide evidence that the native function of Mj0968 seems to be that of a phosphatase, rather than that of an ATP-hydrolyzing enzyme. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofFEBS LETTERS
dc.subject2 `-O-trinitrophenyl nucleotide binding
dc.subjectADENOSINE 5'-TRIPHOSPHATE
dc.subjectBINDING
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCALCIUM-PUMP
dc.subjectCell Biology
dc.subjectCYTOPLASMIC LOOP
dc.subjectESCHERICHIA-COLI
dc.subjectMEMBRANE H+-ATPASE
dc.subjectMethanocaldococcus jannaschii
dc.subjectMETHANOCOCCUS-JANNASCHII
dc.subjectMj0968
dc.subjectNUCLEOTIDES
dc.subjectp-nitrophenyl phosphate hydrolysis
dc.subjectP-type ATPase
dc.subjectphosphatase
dc.subjectphosphorylation
dc.subjectSARCOPLASMIC-RETICULUM
dc.subjectTRIPHOSPHATASE
dc.titleThe Methanocaldococcus jannaschii protein Mj0968 is not a P-type ATPase
dc.typejournal article
dc.identifier.doi10.1016/S0014-5793(03)00372-7
dc.identifier.isiISI:000183121500007
dc.description.volume543
dc.description.issue1-3
dc.description.startpage31
dc.description.endpage36
dc.contributor.orcid0000-0001-9511-4999
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationFEBS Lett.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidAlKa770-
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