Estimation of inhibitory organophosphates with purified pig liver carboxylesterase

DC FieldValueLanguage
dc.contributor.authorHeymann, E
dc.date.accessioned2021-12-23T16:02:29Z-
dc.date.available2021-12-23T16:02:29Z-
dc.date.issued1999
dc.identifier.issn00092797
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5439-
dc.description3th International Meeting on Esterases Reacting with Organophosphorus Compounds, INTER UNVI CTR, DUBROVNIK, CROATIA, APR 15-18, 1998
dc.description.abstractOrganophosphates that inhibit acetylcholineesterase normally also inhibit pig liver carboxylesterase irreversibly. Since this liver esterase is well characterized and easily accessible in large amounts, we propose the use of this enzyme for the quantitation of low concentrations of such organophosphates. The principle of two estimation methods is described. Both methods involve the addition of an unknown amount of organophosphate to an assay mixture bf purified esterase, buffer and a low affinity esterase substrate. In the first of these methods the inhibitor concentration is calculated from the esterase activities before and after the addition of the inhibitor. In the second method, the amounts of inhibitor or of enzyme are changed in several assays, until equimolar conditions can be detected from the observed reaction kinetics. The theoretical background of these methods is discussed and practical examples for the estimation of paraoxon (order of 0.1 nmoles) are given. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
dc.language.isoen
dc.publisherELSEVIER IRELAND LTD
dc.relation.ispartofCHEMICO-BIOLOGICAL INTERACTIONS
dc.subjectBiochemistry & Molecular Biology
dc.subjectESTERASE
dc.subjectkinetics of suicide inhibition
dc.subjectPharmacology & Pharmacy
dc.subjectpig liver carboxylesterase
dc.subjectquantitation of organophosphate inhibitors
dc.subjectquantitation of suicide enzyme inhibitors
dc.subjectToxicology
dc.titleEstimation of inhibitory organophosphates with purified pig liver carboxylesterase
dc.typeconference paper
dc.identifier.doi10.1016/S0009-2797(99)00072-1
dc.identifier.isiISI:000081184400065
dc.description.volume119
dc.description.startpage577
dc.description.endpage586
dc.publisher.placeELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND
dcterms.isPartOf.abbreviationChem.-Biol. Interact.
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