Thylakoid membranes contain a high-conductance channel

DC FieldValueLanguage
dc.contributor.authorHinnah, SC
dc.contributor.authorWagner, R
dc.date.accessioned2021-12-23T16:02:30Z-
dc.date.available2021-12-23T16:02:30Z-
dc.date.issued1998
dc.identifier.issn00142956
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5445-
dc.description.abstractIon channels in the thylakoid membrane were investigated by direct patch clamping on swollen thylakoids. A preparation method has been developed in order to release osmotically swollen intact thylakoids from pea protoplasts derived from cotyledons of young Pisum sativum plants. The swollen thylakoids with typical diameters between 10 mu m and 20 mu m formed reproducibly high-resistance seals with patch pipettes. We observed a potassium channel with a main conductant state of Lambda approximate to 40 pS and a conductance of Lambda approximate to 90 pS (in asymmetric 20/100 mM KCl) for the fully open channel. Surprisingly, the thylakoid membranes also contained a high-conductance channel with a main conductant slate of Lambda approximate to 620 pS (in asymmetric 20/100 mM KCl), revealing also higher and lower conductant states. With a different experimental approach we showed that thylakoids are able to accumulate transiently the membrane impermeant fluorescent dye Lucifer Yellow which likewise suggests the presence of a pore-like channel with a diameter large enough to allow permeation of Lucifer Yellow.
dc.language.isoen
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofEUROPEAN JOURNAL OF BIOCHEMISTRY
dc.subjectBiochemistry & Molecular Biology
dc.subjectchloroplast
dc.subjectDARK
dc.subjectDRIVEN
dc.subjectfluorescence
dc.subjectINHIBITION
dc.subjectINNER MITOCHONDRIAL-MEMBRANE
dc.subjectINTACT CHLOROPLASTS
dc.subjection channel
dc.subjectLIGHT
dc.subjectpatch clamp
dc.subjectPATHWAYS
dc.subjectPH
dc.subjectPHOTOPHOSPHORYLATION
dc.subjectthylakoid
dc.subjectTRANSPORT
dc.titleThylakoid membranes contain a high-conductance channel
dc.typejournal article
dc.identifier.doi10.1046/j.1432-1327.1998.2530606.x
dc.identifier.isiISI:000073524300009
dc.description.volume253
dc.description.issue3
dc.description.startpage606
dc.description.endpage613
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationEur. J. Biochem.
dcterms.oaStatusBronze
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidWaRi703-
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