Precursor and effector dependency of lipid synthesis in amyloplasts isolated from developing maize and wheat endosperm

Autor(en): Mohlmann, T
Neuhaus, HE
Stichwörter: amyloplasts; BIOSYNTHESIS; CAULIFLOWER-BUD PLASTIDS; FATTY-ACID SYNTHESIS; FLORAL BUDS; Food Science & Technology; maize; METABOLITE LEVELS; PEA ROOT PLASTIDS; precursor dependency; PYRUVATE-DEHYDROGENASE COMPLEX; SPINACH-CHLOROPLASTS; STARCH SYNTHESIS; TRANSLOCATORS; wheat
Erscheinungsdatum: 1997
Herausgeber: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Journal: JOURNAL OF CEREAL SCIENCE
Volumen: 26
Ausgabe: 2
Startseite: 161
Seitenende: 167
Zusammenfassung: 
Recently, we developed a method for the isolation of amyloplasts from maize endosperm. Here we describe a new protocol for the isolation of intact and pure amyloplasts From developing wheat endosperm. The precursor and effector dependencies of fatty-acid synthesis were analysed for both types of cereal amyloplasts. Both wheat and maize-endosperm amyloplasts used [2(14)C]pyruvate with the greatest efficiency for the synthesis of fatty acids. Malate was not converted to fatty-acids. The effects of various metabolites upon both pyruvate- and acetate-dependent fatty-acid synthesis were compared for maize or wheat plastids. In all cases, fatty-acid synthesis was dependent upon the additional presence of ATP in the incubation medium. [2(14)C]pyruvate-driven fatty-acid synthesis in isolated wheat amyloplasts was not totally dependent upon the additional presence of reduced pyridine dinucleotide (NADH) in the incubation medium. Both pyruvate- and acetate-dependent fatty-acid synthesis in cereal amyloplasts could be stimulated to maximal rates by exogenous glucose G-phosphate indicating an interaction of the oxidative pentose-phosphate pathway and fatty-acid synthesis. (C) 1997 Academic Press Limited.
ISSN: 07335210
DOI: 10.1006/jcrs.1997.0116

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