Precursor and effector dependency of lipid synthesis in amyloplasts isolated from developing maize and wheat endosperm

DC FieldValueLanguage
dc.contributor.authorMohlmann, T
dc.contributor.authorNeuhaus, HE
dc.date.accessioned2021-12-23T16:02:30Z-
dc.date.available2021-12-23T16:02:30Z-
dc.date.issued1997
dc.identifier.issn07335210
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5451-
dc.description.abstractRecently, we developed a method for the isolation of amyloplasts from maize endosperm. Here we describe a new protocol for the isolation of intact and pure amyloplasts From developing wheat endosperm. The precursor and effector dependencies of fatty-acid synthesis were analysed for both types of cereal amyloplasts. Both wheat and maize-endosperm amyloplasts used [2(14)C]pyruvate with the greatest efficiency for the synthesis of fatty acids. Malate was not converted to fatty-acids. The effects of various metabolites upon both pyruvate- and acetate-dependent fatty-acid synthesis were compared for maize or wheat plastids. In all cases, fatty-acid synthesis was dependent upon the additional presence of ATP in the incubation medium. [2(14)C]pyruvate-driven fatty-acid synthesis in isolated wheat amyloplasts was not totally dependent upon the additional presence of reduced pyridine dinucleotide (NADH) in the incubation medium. Both pyruvate- and acetate-dependent fatty-acid synthesis in cereal amyloplasts could be stimulated to maximal rates by exogenous glucose G-phosphate indicating an interaction of the oxidative pentose-phosphate pathway and fatty-acid synthesis. (C) 1997 Academic Press Limited.
dc.language.isoen
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
dc.relation.ispartofJOURNAL OF CEREAL SCIENCE
dc.subjectamyloplasts
dc.subjectBIOSYNTHESIS
dc.subjectCAULIFLOWER-BUD PLASTIDS
dc.subjectFATTY-ACID SYNTHESIS
dc.subjectFLORAL BUDS
dc.subjectFood Science & Technology
dc.subjectmaize
dc.subjectMETABOLITE LEVELS
dc.subjectPEA ROOT PLASTIDS
dc.subjectprecursor dependency
dc.subjectPYRUVATE-DEHYDROGENASE COMPLEX
dc.subjectSPINACH-CHLOROPLASTS
dc.subjectSTARCH SYNTHESIS
dc.subjectTRANSLOCATORS
dc.subjectwheat
dc.titlePrecursor and effector dependency of lipid synthesis in amyloplasts isolated from developing maize and wheat endosperm
dc.typejournal article
dc.identifier.doi10.1006/jcrs.1997.0116
dc.identifier.isiISI:A1997XZ44600002
dc.description.volume26
dc.description.issue2
dc.description.startpage161
dc.description.endpage167
dc.identifier.eissn10959963
dc.publisher.place24-28 OVAL RD, LONDON NW1 7DX, ENGLAND
dcterms.isPartOf.abbreviationJ. Cereal Sci.
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