Functional expression of the entire adhesiome of Salmonella enterica serotype Typhimurium

Autor(en): Hansmeier, Nicole
Miskiewicz, Katarzyna
Elpers, Laura
Liss, Viktoria
Hensel, Michael 
Sterzenbach, Torsten 
Stichwörter: AGGREGATIVE FIMBRIAE; ATOMIC-FORCE MICROSCOPY; BACTERIAL ADHESION; BIOFILM FORMATION; COLONIZATION FACTOR; ELECTRON-MICROSCOPY; ESCHERICHIA-COLI; I FIMBRIAE; Multidisciplinary Sciences; NON-FIMBRIAL ADHESIN; Science & Technology - Other Topics; TYPE-1 FIMBRIAE
Erscheinungsdatum: 2017
Herausgeber: NATURE PORTFOLIO
Journal: SCIENTIFIC REPORTS
Volumen: 7
Zusammenfassung: 
Adhesins are crucial virulence factors of pathogenic bacteria involved in colonization, transmission and pathogenesis. Many bacterial genomes contain the information for a surprisingly large number of diverse adhesive structures. One prominent example is the invasive and facultative intracellular pathogen Salmonella enterica with an adhesiome of up to 20 adhesins. Such large repertoire of adhesins contributes to colonization of a broad range of host species and may allow adaptation to various environments within the host, as well as in non-host environments. For S. enterica, only few members of the adhesiome are functionally expressed under laboratory conditions, and accordingly the structural and functional understanding of the majority of adhesins is sparse. We have devised a simple and versatile approach to functionally express all adhesins of S. enterica serotype Typhimurium, either within Salmonella or within heterologous hosts such as Escherichia coli. We demonstrate the surface expression of various so far cryptic adhesins and show ultrastructural features using atomic force microscopy and transmission electron microscopy. In summary, we report for the first time the expression of the entire adhesiome of S. enterica serotype Typhimurium.
ISSN: 20452322
DOI: 10.1038/s41598-017-10598-2

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