Baker's yeast as a tool for the development of antifungal kinase inhibitors - targeting protein kinase C and the cell integrity pathway

DC FieldValueLanguage
dc.contributor.authorHeinisch, JJ
dc.date.accessioned2021-12-23T16:02:43Z-
dc.date.available2021-12-23T16:02:43Z-
dc.date.issued2005
dc.identifier.issn15709639
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5570-
dc.description4th International Conference on Inhibitors of Protein Kinases (IPK 2005), Warsaw, POLAND, JUN 25-29, 2005
dc.description.abstractToday, the yeast Saccharomyces cerevisiae is probably the best-studied eukaryotic organism. This review first focusses on the signaling process which is mediated by the unique yeast protein kinase C (Pkc1p) and a downstream mitogen-activated protein kinase (MAPK) cascade. This pathway ensures cellular integrity by sensing cell surface stress and controlling cell wall biosynthesis and progression through the cell cycle. The domain structure of Pkc1p is conserved from yeast to humans. A yeast system for heterologous expression of specific domains in a chimeric yeast/mammalian PKC enzyme (''domain shuffling'') is depicted. It is also proposed how this system could be employed for the study of protein kinase inhibitors in high-throughput screens. Moreover, a reporter assay that allows a quantitative readout of the activity of the cell integrity signaling pathway is introduced. Since a variety of protein kinases take part in the signal transduction, this broadens the range of targets for potential inhibitors. (c) 2005 Elsevier B.V. All rights reserved.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofBIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
dc.subjectACTIN CYTOSKELETON
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCANDIDA-ALBICANS
dc.subjectdomain shuffling
dc.subjectEIF-2-ALPHA KINASE
dc.subjectfungi
dc.subjectGENE-EXPRESSION
dc.subjectKLUYVEROMYCES-LACTIS
dc.subjectMAP KINASE
dc.subjectMOLECULAR-CLONING
dc.subjectreporter system
dc.subjectSaccharomyces cerevisiae
dc.subjectSACCHAROMYCES-CEREVISIAE
dc.subjectsignal transduction
dc.subjectSIGNALING PATHWAY
dc.subjectWALL INTEGRITY
dc.titleBaker's yeast as a tool for the development of antifungal kinase inhibitors - targeting protein kinase C and the cell integrity pathway
dc.typeconference paper
dc.identifier.doi10.1016/j.bbapap.2005.07.032
dc.identifier.isiISI:000234360800017
dc.description.volume1754
dc.description.issue1-2, SI
dc.description.startpage171
dc.description.endpage182
dc.contributor.researcheridG-3801-2017
dc.identifier.eissn00063002
dc.publisher.placePO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dcterms.isPartOf.abbreviationBBA-Proteins Proteomics
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