DNA-FRAGMENTS RESISTANT TO ENDODEOXYRIBONUCLEASE HYDROLYSIS - PCR-AMPLIFICATION USING 7-DEAZA-2'-DEOXYGUANOSINE TRIPHOSPHATE IN PLACE OF DGTP

Autor(en): SEELA, F
ROLING, A
Stichwörter: Biochemistry & Molecular Biology; DIDEOXY
Erscheinungsdatum: 1991
Herausgeber: MARCEL DEKKER INC
Journal: NUCLEOSIDES & NUCLEOTIDES
Volumen: 10
Ausgabe: 1-3
Startseite: 715
Seitenende: 717
Zusammenfassung: 
7-Deaza-2'-deoxyguanosine triphosphate was used instead of dGTP during PCR-amplification of a pUC 18 fragment of 501 base pairs. The oligomer containing c7G(d) was protected from hydrolysis by various endodeoxyribonucleases when the 7-deazapurine nucleoside was localized at the 3'- or the 5'-position of the cleavage site.
Beschreibung: 
9TH INTERNATIONAL ROUND TABLE ON NUCLEOSIDES, NUCLEOTIDES, AND THEIR BIOLOGICAL APPLICATIONS, UPPSALA, SWEDEN, JUL 30-AUG 03, 1990
ISSN: 07328311
DOI: 10.1080/07328319108046580

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