DNA-FRAGMENTS RESISTANT TO ENDODEOXYRIBONUCLEASE HYDROLYSIS - PCR-AMPLIFICATION USING 7-DEAZA-2'-DEOXYGUANOSINE TRIPHOSPHATE IN PLACE OF DGTP

Abstract

7-Deaza-2'-deoxyguanosine triphosphate was used instead of dGTP during PCR-amplification of a pUC 18 fragment of 501 base pairs. The oligomer containing c7G(d) was protected from hydrolysis by various endodeoxyribonucleases when the 7-deazapurine nucleoside was localized at the 3'- or the 5'-position of the cleavage site.