A versatile remote control system for functional expression of bacterial virulence genes based on the tetA promoter

DC FieldValueLanguage
dc.contributor.authorSchulte, Marc
dc.contributor.authorSterzenbach, Torsten
dc.contributor.authorMiskiewicz, Katarzyna
dc.contributor.authorElpers, Laura
dc.contributor.authorHensel, Michael
dc.contributor.authorHansmeier, Nicole
dc.date.accessioned2021-12-23T16:02:55Z-
dc.date.available2021-12-23T16:02:55Z-
dc.date.issued2019
dc.identifier.issn14384221
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5692-
dc.description.abstractThe expression of bacterial virulence factors is controlled in response to host or environmental factors and most virulence genes are not expressed under laboratory conditions. Investigations of molecular structures and cellular functions of bacterial virulence factors demand systems for experimentally controlled expression. We describe a simple and robust system that is based on the tetA promoter and the cognate repressor TetR. Expression under control of P-tetA can be induced by non-antibiotic derivatives of tetracycline such as anhydrotetracycline (AHT). Tet-on expression cassettes can be used to replace native promoters of chromosomal genes or operons of interest. Tet-on plasmids allow episomal expression in homologous or heterologous host organisms. We demonstrate the application of Tet-on systems for the controlled induction of flagella assembly and motility, and for surface expression of adhesins of the chaperone/usher family of enteropathogenic Escherichia coli and autotransporter adhesins of Yersinia enterocolitica in Salmonella enterica and E. coli. Since inducer AHT can easily cross bacterial envelopes and mammalian cell membranes, the system can also be applied to control virulence genes in intracellular bacteria. We demonstrate the controlled synthesis, translocation and function of effector proteins of the type III secretion system of intracellular S. enterica.
dc.description.sponsorshipDeutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SFB944, STE 1921/3-1]; startup grant of the Ministerium fur Wissenschaft und Kultur Lower Saxony; This work was supported by the Deutsche Forschungsgemeinschaft though grants P4 and Z within collaborative research center SFB944 (to M.H.) and STE 1921/3-1 (to T.S.). Further support by a startup grant of the Ministerium fur Wissenschaft und Kultur Lower Saxony to M.H. is kindly acknowledged. T.S. and N.H. received Incentive Awards for the School of Biology/Chemistry. We kindly acknowledge the excellent technical support by Monika Nietschke, Ursula Krehe, Timo Herrenkind and Luca Wallach. We like to thank Andreas Baumler for sharing antisera against fimbrial subunits, Jorge A. Giron for antisera against EcpA, Ingo Autenrieth for YadA and Inv antisera, and Marc Erhardt for sharing antisera against FliC. The authors have declared no conflict of interests.
dc.language.isoen
dc.publisherELSEVIER GMBH
dc.relation.ispartofINTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
dc.subjectADHESION
dc.subjectAutotransporter adhesins
dc.subjectDETECT
dc.subjectENDOSOMAL SYSTEM
dc.subjectFIMBRIAE
dc.subjectFimbrial adhesins
dc.subjectHeterologous expression system
dc.subjectIII SECRETION
dc.subjectINTRACELLULAR SALMONELLA
dc.subjectMicrobiology
dc.subjectPROTEIN
dc.subjectSALMONELLA PATHOGENICITY ISLAND-2
dc.subjectTet repressor
dc.subjectTRANSLOCATED EFFECTORS
dc.subjectType III secretion system
dc.subjectVECTORS
dc.subjectVirology
dc.titleA versatile remote control system for functional expression of bacterial virulence genes based on the tetA promoter
dc.typejournal article
dc.identifier.doi10.1016/j.ijmm.2018.11.001
dc.identifier.isiISI:000460198800006
dc.description.volume309
dc.description.issue1
dc.description.startpage54
dc.description.endpage65
dc.contributor.orcid0000-0001-6604-6253
dc.contributor.orcid0000-0003-4308-2002
dc.identifier.eissn16180607
dc.publisher.placeHACKERBRUCKE 6, 80335 MUNICH, GERMANY
dcterms.isPartOf.abbreviationInt. J. Med. Microbiol.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0001-6604-6253-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidStTo496-
crisitem.author.netidHeMi480-
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