Characterization of amino acid substitutions in KdpA, the K+-binding and -translocating subunit of the KdpFABC complex of Escherichia coli

Autor(en): van der Laan, M
Gassel, M
Altendorf, K 
Stichwörter: ASPARTIC-ACID; ATPASE; Microbiology; MUTAGENESIS; POTASSIUM CHANNELS; PURIFICATION
Erscheinungsdatum: 2002
Herausgeber: AMER SOC MICROBIOLOGY
Journal: JOURNAL OF BACTERIOLOGY
Volumen: 184
Ausgabe: 19
Startseite: 5491
Seitenende: 5494
Zusammenfassung: 
When grown under K+ limitation, Escherichia coli induces the K+-translocating KdpFABC complex. The stimulation of ATPase activity by NH4+ ions was shown for the first time. Substitutions in KdpA, which is responsible for K+ binding and translocation, revealed that enzyme complexes KdpA:G232A and KdpA:G232S have completely lost their cation selectivity.
ISSN: 00219193
DOI: 10.1128/JB.184.19.5491-5494.2002

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