Characterization of amino acid substitutions in KdpA, the K+-binding and -translocating subunit of the KdpFABC complex of Escherichia coli
| Autor(en): | van der Laan, M Gassel, M Altendorf, K |
Stichwörter: | ASPARTIC-ACID; ATPASE; Microbiology; MUTAGENESIS; POTASSIUM CHANNELS; PURIFICATION | Erscheinungsdatum: | 2002 | Herausgeber: | AMER SOC MICROBIOLOGY | Enthalten in: | JOURNAL OF BACTERIOLOGY | Band: | 184 | Ausgabe: | 19 | Startseite: | 5491 | Seitenende: | 5494 | Zusammenfassung: | When grown under K+ limitation, Escherichia coli induces the K+-translocating KdpFABC complex. The stimulation of ATPase activity by NH4+ ions was shown for the first time. Substitutions in KdpA, which is responsible for K+ binding and translocation, revealed that enzyme complexes KdpA:G232A and KdpA:G232S have completely lost their cation selectivity. |
ISSN: | 00219193 | DOI: | 10.1128/JB.184.19.5491-5494.2002 |
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