CYSTEINES OF CHLOROPLAST NADP-MALATE DEHYDROGENASE FORM MIXED DISULFIDES

DC FieldValueLanguage
dc.contributor.authorOCHERETINA, O
dc.contributor.authorSCHEIBE, R
dc.date.accessioned2021-12-23T16:03:04Z-
dc.date.available2021-12-23T16:03:04Z-
dc.date.issued1994
dc.identifier.issn18733468
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5775-
dc.description.abstractChloroplast NADP-malate dehydrogenase (NADP-MDH) from pea and from spinach was N-terminally truncated by limited proteolysis with Staphylococcus aureus protease V8. The resulting monomeric enzymes lacking, respectively, the 37 and 38 N-terminal amino acids were inactive. Reduction and addition of low concentrations of guanidine-HCl (50-100 mM) resulted in a highly active enzyme of 850 units per mg protein. Equilibration of the truncated enzyme with various glutathione (GSH) redox buffers and assaying its activity in the presence of guanidine-HCl was used to establish the existence of protein-GSH mixed disulfides. This finding was further confirmed using incorporation of radioactively labelled thiol. The possible function of such cysteine modifications under oxidative stress and their regeneration by the thioredoxin system in the light is discussed.
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofFEBS LETTERS
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCell Biology
dc.subjectCLONING
dc.subjectDEPENDENT REGULATORY SITE
dc.subjectDIRECTED MUTAGENESIS
dc.subjectEXPRESSION
dc.subjectGLUTATHIONE
dc.subjectLIMITED PROTEOLYSIS
dc.subjectMIXED DISULFIDE
dc.subjectNADP-MALATE DEHYDROGENASE
dc.subjectOXIDATIVE MODIFICATION
dc.subjectOXIDATIVE STRESS
dc.subjectPEA
dc.subjectPEA CHLOROPLAST
dc.subjectPURIFICATION
dc.subjectREDOX MODIFICATION
dc.subjectREDUCTIVE ACTIVATION
dc.subjectSEQUENCE
dc.subjectSTAPHYLOCOCCUS AUREUS PROTEASE V8
dc.titleCYSTEINES OF CHLOROPLAST NADP-MALATE DEHYDROGENASE FORM MIXED DISULFIDES
dc.typejournal article
dc.identifier.doi10.1016/0014-5793(94)01214-8
dc.identifier.isiISI:A1994PW26400010
dc.description.volume355
dc.description.issue3
dc.description.startpage254
dc.description.endpage258
dc.publisher.place111 RIVER ST, HOBOKEN 07030-5774, NJ USA
dcterms.isPartOf.abbreviationFEBS Lett.
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.orcid0000-0002-6140-6181-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScRe288-
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