The stoichiometry of subunit c of Escherichia coli ATP synthase is independent of its rate of synthesis
Autor(en): | Krebstakies, Thomas Aldag, Ingo Altendorf, Karlheinz Greie, Joerg-Christian Deckers-Hebestreit, Gabriele |
Stichwörter: | BINDING SITE MUTATION; Biochemistry & Molecular Biology; CROSS-LINKING; F-0 COMPLEX; F1F0 ATPASE; H+/ATP RATIO; OLIGOMER; PROTON-TRANSLOCATING ATPASE; RECONSTITUTION; ROTOR RING; SECTOR | Erscheinungsdatum: | 2008 | Herausgeber: | AMER CHEMICAL SOC | Journal: | BIOCHEMISTRY | Volumen: | 47 | Ausgabe: | 26 | Startseite: | 6907 | Seitenende: | 6916 | Zusammenfassung: | Immunoblot quantitation of Escherichia coli ATP synthase isolated from atp wildtype and mutant cells, the latter comprising a reduced expression of the atpE gene coding for subunit c due to a point mutation within its Shine-Dalgarno sequence, suggested a variable stoichiometry of subunit c [Schemidt et al. (1995) Arch. Biochem. Biophys. 323, 423-428]. To study the c ring of the mutant strains and its stoichiometry in more detail, F(O) isolated. from wildtype and mutant were investigated by quantitation, reconstitution, and cross-linking. Direct quantitation by staining with SYPRO Ruby revealed a reduction of subunit c in the mutant by a factor of 2 compared to F(O) subunits a and b. Rates of passive H(+) translocation correlated with the amount of subunit c present. Lower rates for mutant F(O) could be increased by addition of subunit c, whereas translocation rates remained constant by coreconstitution with nonfunctional subunit cD61G arguing against the presence of smaller c rings that are filled up with coreconstituted subunit c. Intermolecular cross-linking by oxidation of bicysteine-substituted subunit c (cA21C/cM65C) revealed an equal pattern of oligomer formation in wildtype and mutant also favoring a comparable subunit c stoichiometry. Cross-linking of membrane vesicles containing cysteine-substituted subunits a (aN214C) and c (cM65C) characterized the mutant FO preparation as a heterogeneous population, which consists of assembled F(O) and free ab(2) subcomplexes each present to approximately 50%. Thus, these data clearly demonstrate that the stoichiometry of the subunit c rings remains constant even after reduction of the synthesis of subunit c. |
ISSN: | 00062960 | DOI: | 10.1021/bi800173a |
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