The novel extracellular Streptomyces reticuli haem-binding protein HbpS influences the production of the catalase-peroxidase CpeB

DC FieldValueLanguage
dc.contributor.authorLucana, DOD
dc.contributor.authorSchaa, T
dc.contributor.authorSchrempf, H
dc.date.accessioned2021-12-23T16:03:18Z-
dc.date.available2021-12-23T16:03:18Z-
dc.date.issued2004
dc.identifier.issn13500872
dc.identifier.urihttps://osnascholar.ub.uni-osnabrueck.de/handle/unios/5912-
dc.description.abstractThe Gram-positive soil bacterium and cellulose degrader Streptomyces reticuli synthesizes the mycelium-associated enzyme CpeB, which displays haem-dependent catalase and peroxidase activity, as well as haem-independent manganese-peroxidase activity. Downstream of the cpeB gene, a so far unknown gene was identified. The new gene and its mutated derivatives were cloned in Escherichia coli as well as in Streptomyces lividans and a gene-disruption mutant within the chromosome of the original S. reticuli host was constructed, comparative physiological, biochemical and immunological studies then allowed the deduction of the following characteristics of the novel gene product. (i) The protein was found extracellularly; the substitution of twin arginines within the signal peptide abolished its secretion. (ii) The highly purified protein interacted specifically with haem and hence was designated HbpS (haem-binding protein of Streptomyces). (iii) HbpS contained three histidine residues surrounded by hydrophobic amino acids; one of them was located within the motif LX(3)THLX(10)AA, which is related to the motif within the yeast cytochrome c peroxidase LX(2)THLX(10)AA whose histidine residue interacts with haem. (iv) The addition of haemin (Fe3+ oxidized form of haem) to the Streptomyces cultures led to enhanced levels of HbpS which correlated with increased haemin-resistance. (v) The presence of HbpS increased synthesis of the highly active catalase-peroxidase CpeB containing haem. In this process HbpS could act as a chaperone that binds haem and then delivers it to the mycelium-associated CpeB; HbpS could also interact with membrane-associated proteins involved in a signal transduction cascade regulating the expression of cpeB. (vi) HbpS shared varying degrees of amino acid identities with bacterial proteins of so far unknown function. This report contributes to the elucidation of the biological function of these proteins.
dc.language.isoen
dc.publisherMICROBIOLOGY SOC
dc.relation.ispartofMICROBIOLOGY-SGM
dc.subjectARGININE TRANSLOCATION PATHWAY
dc.subjectBIOSYNTHESIS
dc.subjectCELLULASE AVICELASE
dc.subjectESCHERICHIA-COLI
dc.subjectEXPORT PATHWAY
dc.subjectGENES
dc.subjectGENOME
dc.subjectIDENTIFICATION
dc.subjectMicrobiology
dc.subjectREGULATOR FURS
dc.subjectSENSITIVE METHOD
dc.titleThe novel extracellular Streptomyces reticuli haem-binding protein HbpS influences the production of the catalase-peroxidase CpeB
dc.typejournal article
dc.identifier.doi10.1099/mic.0.27091-0
dc.identifier.isiISI:000223365600011
dc.description.volume150
dc.description.issue8
dc.description.startpage2575
dc.description.endpage2585
dc.identifier.eissn14652080
dc.publisher.placeCHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND
dcterms.isPartOf.abbreviationMicrobiology-(UK)
crisitem.author.deptFB 05 - Biologie/Chemie-
crisitem.author.deptidfb05-
crisitem.author.parentorgUniversität Osnabrück-
crisitem.author.netidScHi752-
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