Exploring the open pore of the potassium channel from Streptomyces lividans
Autor(en): | Meuser, D Splitt, H Wagner, R Schrempf, H |
Stichwörter: | Biochemistry & Molecular Biology; Biophysics; BLOCK; Cell Biology; CONDUCTION; ION CHANNEL; ion permeation; KcsA; ORGANIC CATIONS; PERMEABILITY; pore; potassium channel; RECTIFICATION; RECTIFIER K+ CHANNEL; SARCOPLASMIC-RETICULUM; SELECTIVITY; Streptomyces lividans; TETRAETHYLAMMONIUM | Erscheinungsdatum: | 1999 | Herausgeber: | ELSEVIER SCIENCE BV | Enthalten in: | FEBS LETTERS | Band: | 462 | Ausgabe: | 3 | Startseite: | 447 | Seitenende: | 452 | Zusammenfassung: | The tetrameric potassium channel from Streptomyces lividans (KcsA) embedded in planar bilayers exhibits the following electrophysiological characteristics: (i) K+ ions can cross the pore in a highly hydrated state (n(H2O) greater than or similar to 6), (ii) the selectivity for K+ exceeds that for Na+ ions by 11 times, and both Ca2+ and Mg2+ are permeant, (iii) the internal side is blocked by Ba2+ ions in a voltage-dependent manner, (iv) intrinsic rectification is due to gating, depending on the direction of the electric field, (v) the internal side is pH-sensitive, and (vi) the open pore has a diameter of similar to 5.8 Angstrom. In conclusion, our results show that ion conduction and selectivity of KcsA cannot easily be reconciled with the properties deduced from the rigid crystal structure [Doyle et al,, Science 280 (1998) 69-77], which must be concluded to have the pore trapped in its closed state. (C) 1999 Federation of European Biochemical Societies. |
ISSN: | 00145793 | DOI: | 10.1016/S0014-5793(99)01579-3 |
Zur Langanzeige
Seitenaufrufe
2
Letzte Woche
1
1
Letzter Monat
1
1
geprüft am 06.06.2024